Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SCD Double Nickase Plasmid (h): sc-401516-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCD Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SCD Double Nickase Plasmid (h) and SCD Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SCD. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCD Antibody (D-5): sc-515875
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCD Double Nickase Plasmid (h) | sc-401516-NIC | 20 µg | $410.00 | |||
SCD Double Nickase Plasmid (h2) | sc-401516-NIC-2 | 20 µg | $410.00 |
Stearoyl-CoA desaturase (SCD) is a key endoplasmic reticulum enzyme that introduces a cis double bond into saturated fatty acyl-CoAs, generating monounsaturated fatty acids that support membrane fluidity, lipid droplet biogenesis, and protein acylation. By controlling the saturated-to-monounsaturated lipid balance, SCD influences de novo lipogenesis, phospholipid remodeling, and signaling networks linked to ER stress and oxidative stress. SCD activity integrates with SREBP- and PPAR-regulated metabolic programs that coordinate fatty acid synthesis and storage. Dysregulated SCD expression or activity is associated with metabolic phenotypes involving insulin sensitivity and hepatic lipid accumulation and is frequently studied in contexts of lipid-dependent growth and stress adaptation.
SCD Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SCD locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SCD. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SCD function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SCD-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.