Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SCCA1 Double Nickase Plasmid (h): sc-403601-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCCA1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SCCA1 Double Nickase Plasmid (h) and SCCA1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SERPINB3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCCA1 Antibody (8H11): sc-21767
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCCA1 Double Nickase Plasmid (h) | sc-403601-NIC | 20 µg | $410.00 | |||
SCCA1 Double Nickase Plasmid (h2) | sc-403601-NIC-2 | 20 µg | $410.00 |
SERPINB3 encodes squamous cell carcinoma antigen 1 (SCCA1), a clade B serpin that functions primarily as an intracellular protease inhibitor. SCCA1 modulates protease-driven processes linked to epithelial differentiation, inflammatory responses, and cellular stress handling, including protection from lysosomal and cytosolic protease activity. By shaping proteostasis and cell survival signaling, SERPINB3 is frequently studied in contexts of epithelial remodeling and dysregulated immune microenvironments. Altered SCCA1 expression has been associated with squamous epithelial pathology and cancer-associated phenotypes, supporting its use as a mechanistic marker in disease-relevant models.
SCCA1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SERPINB3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SERPINB3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SERPINB3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SERPINB3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.