Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SCCA1/2 Double Nickase Plasmid (h): sc-418018-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCCA1/2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SCCA1/2 Double Nickase Plasmid (h) and SCCA1/2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SERPINB4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCCA1/2 Antibody (B-9): sc-28384
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCCA1/2 Double Nickase Plasmid (h) | sc-418018-NIC | 20 µg | $410.00 |
SERPINB4 encodes squamous cell carcinoma antigen 1/2 (SCCA1/2), a clade B serine protease inhibitor that modulates pericellular and intracellular proteolysis by targeting enzymes such as cathepsins and related proteases. By constraining protease activity, SCCA1/2 influences epithelial barrier homeostasis, inflammatory responses, and stress-associated processes including apoptosis, lysosomal function, and remodeling of the extracellular milieu. SERPINB4 expression is enriched in stratified epithelia and can be altered in settings of chronic inflammation and epithelial dysplasia, making it a useful molecular node for studying protease–antiprotease balance. Dysregulated SCCA1/2 has been associated with changes in tumor-adjacent microenvironments and immune signaling networks, supporting its relevance in mechanistic studies of epithelial pathobiology.
SCCA1/2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SERPINB4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SERPINB4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SERPINB4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SERPINB4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.