SCARA5 CRISPR/Cas9 KO Plasmid (h): sc-407005
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCARA5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCARA5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCARA5 CRISPR/Cas9 KO Plasmid (h) | sc-407005 | 20 µg | $397.00 |
Overview
SCARA5 (scavenger receptor class A member 5) is a cell-surface pattern recognition receptor implicated in the binding and internalization of specific ligands, including ferritin, linking it to iron handling and receptor-mediated endocytosis. In human cells, SCARA5 contributes to regulation of cellular adhesion and migration and can influence inflammatory signaling outputs through scavenger receptor–associated processes. Altered SCARA5 expression has been reported across multiple tumor contexts and in fibrotic or inflammatory microenvironments, where it may correlate with changes in epithelial–mesenchymal programs and extracellular matrix remodeling. These features make SCARA5 a useful target for mechanistic studies of iron-associated signaling, membrane trafficking, and microenvironmental regulation of cell behavior.
SCARA5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SCARA5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SCARA5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SCARA5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCARA5 protein expression.
This CRISPR knockout system enables efficient generation of SCARA5-deficient cell models for investigation of SCARA5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SCARA5 exon(s) critical for SCARA5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SCARA5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCARA5 CRISPR/Cas9 KO Plasmid (h) and SCARA5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SCARA5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCARA5 HDR Plasmid (h) and SCARA5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SCARA5 homology arms to support homology-directed repair at defined SCARA5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.