SATB2 CRISPR/Cas9 KO Plasmid (m): sc-431818
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SATB2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SATB2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SATB2 Antibody (SATBA4B10): sc-81376
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SATB2 CRISPR/Cas9 KO Plasmid (m) | sc-431818 | 20 µg | $397.00 |
Overview
Satb2 encodes SATB2, a nuclear matrix-associated transcriptional regulator that binds AT-rich DNA elements and organizes higher-order chromatin structure to coordinate lineage-specific gene expression programs in mouse cells. SATB2 influences developmental transcriptional networks and epigenetic regulation, impacting processes such as differentiation, tissue patterning, and neuronal maturation through modulation of chromatin accessibility and long-range enhancer–promoter interactions. In the immune system and other tissues, SATB family proteins integrate signaling-dependent transcriptional responses by shaping genome architecture and co-regulator recruitment. Dysregulated SATB2 activity is associated with altered differentiation states and aberrant gene expression signatures relevant to developmental phenotypes and disease-associated transcriptional rewiring.
SATB2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Satb2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Satb2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Satb2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SATB2 protein expression.
This CRISPR knockout system enables efficient generation of Satb2-deficient cell models for investigation of SATB2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Satb2 exon(s) critical for SATB2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Satb2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SATB2 CRISPR/Cas9 KO Plasmid (m) and SATB2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Satb2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SATB2 HDR Plasmid (m) and SATB2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Satb2 homology arms to support homology-directed repair at defined Satb2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.