SASH1 CRISPR/Cas9 KO Plasmid (h): sc-403567
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SASH1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SASH1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SASH1 Antibody (X1): sc-517001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SASH1 CRISPR/Cas9 KO Plasmid (h) | sc-403567 | 20 µg | $397.00 |
Overview
SASH1 (SAM and SH3 domain containing 1) encodes an adaptor/scaffold protein that integrates signaling through SAM and SH3 interaction modules to coordinate cytoskeletal organization, cell adhesion, and migratory behavior. It has been linked to regulation of MAPK/ERK and other kinase-driven pathways that shape epithelial integrity, stress responses, and cell fate decisions. Altered SASH1 expression or function has been reported across multiple cancer types and is frequently studied in the context of invasion, metastasis-associated phenotypes, and tumor suppressor-like signaling networks. SASH1 is also of interest in vascular and inflammatory biology, where adaptor-mediated signaling can influence endothelial function and barrier properties.
SASH1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SASH1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SASH1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SASH1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SASH1 protein expression.
This CRISPR knockout system enables efficient generation of SASH1-deficient cell models for investigation of SASH1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SASH1 exon(s) critical for SASH1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SASH1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SASH1 CRISPR/Cas9 KO Plasmid (h) and SASH1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SASH1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SASH1 HDR Plasmid (h) and SASH1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SASH1 homology arms to support homology-directed repair at defined SASH1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.