Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sarcospan CRISPR/Cas9 KO Plasmid (m): sc-421332
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sarcospan CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sarcospan genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sarcospan Antibody (E-2): sc-393187
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sarcospan CRISPR/Cas9 KO Plasmid (m) | sc-421332 | 20 µg | $397.00 |
Overview
Sspn encodes sarcospan, a tetraspan membrane protein that forms part of the dystrophin–glycoprotein complex and helps organize the sarcolemma by stabilizing laminin-binding receptor assemblies. In skeletal and cardiac muscle, sarcospan supports membrane integrity during contraction and contributes to mechanotransduction by influencing the distribution and function of associated sarcoglycans and integrins. Altered sarcospan-dependent complex assembly has been linked to muscle fragility and impaired regeneration, making Sspn a useful entry point for studying pathways relevant to muscular dystrophy phenotypes. Because sarcospan also impacts extracellular matrix interactions and membrane microdomain organization, it is frequently examined in models of myofiber damage, inflammation, and remodeling.
Sarcospan CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sspn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sspn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sspn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sarcospan protein expression.
This CRISPR knockout system enables efficient generation of Sspn-deficient cell models for investigation of Sarcospan signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sspn exon(s) critical for Sarcospan function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sspn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sarcospan CRISPR/Cas9 KO Plasmid (m) and Sarcospan CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sspn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sarcospan HDR Plasmid (m) and Sarcospan HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sspn homology arms to support homology-directed repair at defined Sspn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.