Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sar1a Double Nickase Plasmid (h): sc-404190-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sar1a Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Sar1a Double Nickase Plasmid (h) and Sar1a Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SAR1A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sar1a Antibody (K-44): sc-130463
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sar1a Double Nickase Plasmid (h) | sc-404190-NIC | 20 µg | $410.00 | |||
Sar1a Double Nickase Plasmid (h2) | sc-404190-NIC-2 | 20 µg | $410.00 |
SAR1A encodes Sar1a, a small COPII GTPase that initiates coat assembly at endoplasmic reticulum exit sites to drive ER-to-Golgi transport. By cycling between GDP- and GTP-bound states, Sar1a recruits Sec23/24 and promotes vesicle budding, linking secretory trafficking to membrane homeostasis and proteostasis. SAR1A-dependent COPII dynamics influence secretion of extracellular matrix components and lipoproteins, and perturbations in early secretory pathway function are relevant to cellular stress responses and disorders involving defective protein trafficking. In human cell models, Sar1a is frequently studied for its roles in secretory pathway regulation, organelle organization, and the downstream impact on signaling and metabolism.
Sar1a Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SAR1A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SAR1A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SAR1A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SAR1A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.