SAP Double Nickase Plasmid (h): sc-402165-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SAP Double Nickase Plasmid (h) and SAP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APCS. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SAP Antibody (C-11): sc-393948
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP Double Nickase Plasmid (h) | sc-402165-NIC | 20 µg | $410.00 | |||
SAP Double Nickase Plasmid (h2) | sc-402165-NIC-2 | 20 µg | $410.00 |
APCS encodes serum amyloid P component (SAP), a pentraxin family pattern-recognition protein that circulates in plasma and binds ligands in a calcium-dependent manner, including DNA, chromatin, and microbial polysaccharides. SAP contributes to innate immune surveillance and opsonization by engaging complement-associated processes and Fc receptor–mediated clearance pathways, and it can associate with extracellular matrix components during inflammatory responses. Through its avid binding to amyloid fibrils and other misfolded protein aggregates, SAP is widely used as a molecular handle to study amyloid biology, protein deposition, and persistence of extracellular aggregates in tissues. Altered SAP interactions and APCS-linked regulatory changes have been investigated in the context of systemic inflammation and amyloid-associated pathologies, supporting mechanistic studies of immune-complex handling and aggregate stabilization.
SAP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APCS locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APCS. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APCS function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APCS-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.