Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SAP 155 Double Nickase Plasmid (h): sc-402925-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAP 155 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SAP 155 Double Nickase Plasmid (h) and SAP 155 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SF3B1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SAP 155 Antibody (B-3): sc-514655
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP 155 Double Nickase Plasmid (h) | sc-402925-NIC | 20 µg | $410.00 | |||
SAP 155 Double Nickase Plasmid (h2) | sc-402925-NIC-2 | 20 µg | $410.00 |
SF3B1 encodes SAP 155, an essential core component of the SF3b subcomplex within the U2 snRNP that helps define the branch point and 3′ splice site during pre-mRNA splicing. Through its role in spliceosome assembly and splice site choice, SAP 155 influences transcript isoform diversity and links RNA processing to cell-cycle control, DNA damage responses, and proteostasis. Dysregulation of SF3B1-dependent splicing has been associated with widespread aberrant exon usage and altered gene expression programs across multiple cancers and hematologic malignancies, as well as other disorders where spliceosomal fidelity is compromised. As a result, SF3B1/SAP 155 is frequently studied to connect splicing-factor perturbation with downstream changes in signaling pathways and RNA-seq splicing signatures.
SAP 155 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SF3B1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SF3B1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SF3B1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SF3B1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.