SAMD4A CRISPR/Cas9 KO Plasmid (h): sc-406449
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAMD4A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SAMD4A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAMD4A CRISPR/Cas9 KO Plasmid (h) | sc-406449 | 20 µg | $397.00 |
Overview
SAMD4A (sterile alpha motif domain containing 4A) is an RNA-binding regulatory protein implicated in post-transcriptional control of gene expression through recognition of specific motifs in target mRNAs. It participates in mRNA stability, localization, and translational repression, linking SAMD4A activity to cell-state transitions and stress-adaptive programs that remodel the proteome. Through these functions, SAMD4A can influence pathways governing proliferation, differentiation, and immune-related signaling outputs by modulating the translation of pathway components. Altered SAMD4A regulation has been explored in the context of cancer-associated gene expression programs and inflammatory phenotypes where dysregulated RNA metabolism contributes to disease-relevant cellular behavior.
SAMD4A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SAMD4A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SAMD4A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SAMD4A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SAMD4A protein expression.
This CRISPR knockout system enables efficient generation of SAMD4A-deficient cell models for investigation of SAMD4A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SAMD4A exon(s) critical for SAMD4A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SAMD4A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SAMD4A CRISPR/Cas9 KO Plasmid (h) and SAMD4A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SAMD4A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SAMD4A HDR Plasmid (h) and SAMD4A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SAMD4A homology arms to support homology-directed repair at defined SAMD4A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.