Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SAHH Double Nickase Plasmid (h): sc-402617-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAHH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SAHH Double Nickase Plasmid (h) and SAHH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AHCY. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SAHH Antibody (A-11): sc-271389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAHH Double Nickase Plasmid (h) | sc-402617-NIC | 20 µg | $410.00 | |||
SAHH Double Nickase Plasmid (h2) | sc-402617-NIC-2 | 20 µg | $410.00 |
AHCY encodes S-adenosylhomocysteine hydrolase (SAHH), a conserved enzyme that hydrolyzes S-adenosylhomocysteine to adenosine and homocysteine, thereby relieving product inhibition of S-adenosylmethionine–dependent methyltransferases. By controlling intracellular SAH levels, SAHH supports methylation-dependent regulation of DNA, RNA, protein, and lipid metabolism and influences one-carbon and methionine cycle flux. Perturbation of AHCY activity can shift cellular methylation potential, affecting epigenetic state, transcriptional programs, and metabolite homeostasis. Dysregulated SAH/adenosine balance and altered methylation dynamics have been associated with metabolic and neurologic phenotypes and are frequently examined in studies of redox stress and liver-related biology.
SAHH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AHCY locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AHCY. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AHCY function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AHCY-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.