SAHH Antibody (F-11): sc-365070

SAHH Antibody (F-11) is a mouse monoclonal IgG1 antibody that detects S-adenosylhomocysteine hydrolase (SAHH) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAHH is a crucial cytoplasmic protein composed of 432 amino acids, primarily localized to the melanosome, an organelle responsible for melanin production. SAHH (F-11) antibody recognizes a protein that plays a vital role in amino acid biosynthesis by catalyzing the reversible hydrolysis of S-adenosylhomocysteine (SAH) into adenosine and L-homocysteine, thereby regulating intracellular SAH levels and influencing methyltransferase reactions essential for various biological processes. The homotetrameric structure of SAHH allows binding of one NAD molecule per subunit, facilitating involvement in the activated methyl cycle, where the methyl group of methionine is activated through the formation of S-adenosylmethionine. Mutations in the gene encoding SAHH can lead to hypermethioninemia, a metabolic disorder characterized by elevated methionine levels, resulting in symptoms such as sluggishness, muscle weakness, and liver dysfunction. Anti-SAHH antibody (F-11) serves as an invaluable tool for researchers studying the functional dynamics of SAHH and its implications in health and disease.

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SAHH Antibody (F-11) References:

1. Functional analysis of human S-adenosylhomocysteine hydrolase isoforms SAHH-2 and SAHH-3.  |  Fumić, K., et al. 2007. Eur J Hum Genet. 15: 347-51. PMID: 17164794
2. Mice deficient in cystathionine beta synthase display increased Dyrk1A and SAHH activities in brain.  |  Planque, C., et al. 2013. J Mol Neurosci. 50: 1-6. PMID: 22700376
3. S-adenosyl homocysteine hydrolase (SAHH) accelerates flagellar regeneration in Dunaliella salina.  |  Li, Q., et al. 2013. Curr Microbiol. 67: 249-54. PMID: 23532254
4. Overexpression of S-adenosylhomocysteine hydrolase (SAHH) in esophageal squamous cell carcinoma (ESCC) cell lines: effects on apoptosis, migration and adhesion of cells.  |  Li, Q., et al. 2014. Mol Biol Rep. 41: 2409-17. PMID: 24430301
5. Long non-coding RNA H19/SAHH axis epigenetically regulates odontogenic differentiation of human dental pulp stem cells.  |  Zeng, L., et al. 2018. Cell Signal. 52: 65-73. PMID: 30165103
6. Reversible SAHH inhibitor protects against glomerulonephritis in lupus-prone mice by downregulating renal α-actinin-4 expression and stabilizing integrin-cytoskeleton linkage.  |  He, S., et al. 2019. Arthritis Res Ther. 21: 40. PMID: 30696480
7. SAHH and SAMS form a methyl donor complex with CCoAOMT7 for methylation of phenolic compounds.  |  Yang, SX., et al. 2019. Biochem Biophys Res Commun. 520: 122-127. PMID: 31582217
8. Comparing recombinant MPB70/SahH and native 20-kDa protein for detecting bovine tuberculosis using ELISA.  |  Cho, YS., et al. 2020. J Vet Med Sci. 82: 1631-1638. PMID: 33055466
9. Family and population studies of SAHH and ADA polymorphisms. A possible pitfall in the ascertainment of SAHH electrophoretic phenotypes.  |  Scozzari, R., et al. 1987. Ann Hum Genet. 51: 295-302. PMID: 3447513
10. Dysmorphic Findings in SAHH Deficiency with a Novel Variant in the AHCY Gene.  |  Ciki, K. and Alavanda, C. 2024. Mol Syndromol. 15: 531-536. PMID: 39634240

The preferred SAHH antibody is SAHH Antibody (A-11): sc-271389.