Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SA-1 CRISPR/Cas9 KO Plasmid (m): sc-423170
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SA-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SA-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SA-1 Antibody (A-9): sc-365061
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SA-1 CRISPR/Cas9 KO Plasmid (m) | sc-423170 | 20 µg | $397.00 |
Overview
Mouse Stag1 encodes SA-1, a core component of the cohesin complex that controls sister chromatid cohesion, higher-order chromatin organization, and accurate chromosome segregation during mitosis and meiosis. SA-1 contributes to genome stability and transcriptional regulation by modulating long-range chromatin looping and coordinating DNA replication and repair processes. Through these functions, STAG1 intersects with cell-cycle checkpoints and DNA damage response pathways, making it relevant to studies of aneuploidy, chromosomal instability, and cohesin-associated developmental phenotypes. Disruption or dysregulation of cohesin subunits, including SA-1, is frequently investigated in the context of altered gene expression programs and tumor biology without implying clinical outcomes.
SA-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Stag1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stag1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stag1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SA-1 protein expression.
This CRISPR knockout system enables efficient generation of Stag1-deficient cell models for investigation of SA-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stag1 exon(s) critical for SA-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stag1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SA-1 CRISPR/Cas9 KO Plasmid (m) and SA-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stag1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SA-1 HDR Plasmid (m) and SA-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stag1 homology arms to support homology-directed repair at defined Stag1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.