RuvC Antibody (5G9/3): sc-53437
- RuvC Antibody (5G9/3) is a mouse monoclonal IgG2a κ RuvC antibody, cited in 3 publications, provided at 200 µg/ml
- raised against Ruv C protein from E. coli
- RuvC Antibody (5G9/3) is recommended for detection of RuvC of E. coli origin by WB and IP
- Anti-RuvC Antibody (5G9/3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for RuvC Antibody (5G9/3) for WB applications. This reagent is now offered in a bundle with RuvC Antibody (5G9/3) (see ordering information below).
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RuvC Antibody (5G9/3) is a mouse monoclonal IgG2a kappa light chain antibody that detects the RuvC protein of E. coli origin by western blotting (WB) and immunoprecipitation (IP). RuvC (5G9/3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The RuvC protein plays a critical role in the resolution of Holliday junctions through endonucleolytic cleavage, which is essential for the proper completion of homologous recombination and DNA repair processes. This function is particularly important as RuvC ensures genomic stability by facilitating the accurate repair of double-strand breaks, which can arise from various cellular stresses or replication errors. In Escherichia coli, RuvC works in concert with RuvA and RuvB proteins, forming a complex that not only promotes branch migration but also interacts with damaged DNA to facilitate repair. The RuvABC complex is vital for maintaining the integrity of the genetic material, especially at replication forks where double-strand breaks can lead to severe genomic instability if not properly resolved. Anti-RuvC antibody (5G9/3) enables researchers to effectively study the dynamics of DNA repair mechanisms and RuvC′s role in maintaining cellular health in E. coli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RuvC Antibody (5G9/3) References:
- Two basic residues, Lys-107 and Lys-118, of RuvC resolvase are involved in critical contacts with the Holliday junction for its resolution. | Yoshikawa, M., et al. 2000. Genes Cells. 5: 803-13. PMID: 11029656
- Ensuring productive resolution by the junction-resolving enzyme RuvC: large enhancement of the second-strand cleavage rate. | Fogg, JM. and Lilley, DM. 2000. Biochemistry. 39: 16125-34. PMID: 11123941
- Evidence that phenylalanine 69 in Escherichia coli RuvC resolvase forms a stacking interaction during binding and destabilization of a Holliday junction DNA substrate. | Yoshikawa, M., et al. 2001. J Biol Chem. 276: 10432-6. PMID: 11152689
- p53 blocks RuvAB promoted branch migration and modulates resolution of Holliday junctions by RuvC. | Prabhu, VP., et al. 2002. J Mol Biol. 316: 1023-32. PMID: 11884140
- Parallel symmetric immobile DNA junctions as substrates for E. coli RuvC Holliday junction resolvase. | Sha, R., et al. 2002. Biochemistry. 41: 10985-93. PMID: 12206670
- Helicobacter pylori mutants defective in RuvC Holliday junction resolvase display reduced macrophage survival and spontaneous clearance from the murine gastric mucosa. | Loughlin, MF., et al. 2003. Infect Immun. 71: 2022-31. PMID: 12654822
- Evolution of a phage RuvC endonuclease for resolution of both Holliday and branched DNA junctions. | Curtis, FA., et al. 2005. Mol Microbiol. 55: 1332-45. PMID: 15720544
- Piv site-specific invertase requires a DEDD motif analogous to the catalytic center of the RuvC Holliday junction resolvases. | Buchner, JM., et al. 2005. J Bacteriol. 187: 3431-7. PMID: 15866929
- In vitro reconstitution of the late steps of genetic recombination in E. coli. | Eggleston, AK., et al. 1997. Cell. 89: 607-17. PMID: 9160752
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RuvC Antibody (5G9/3) | sc-53437 | 200 µg/ml | $322.00 | |||
RuvC Antibody (5G9/3): m-IgG Fc BP-HRP Bundle | sc-525594 | 200 µg Ab; 10 µg BP | $361.00 | |||
RuvC Antibody (5G9/3) AC | sc-53437 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RuvC Antibody (5G9/3) HRP | sc-53437 HRP | 200 µg/ml | $322.00 | |||
RuvC Antibody (5G9/3) FITC | sc-53437 FITC | 200 µg/ml | $336.00 | |||
RuvC Antibody (5G9/3) PE | sc-53437 PE | 200 µg/ml | $349.00 | |||
RuvC Antibody (5G9/3) Alexa Fluor® 488 | sc-53437 AF488 | 200 µg/ml | $364.00 | |||
RuvC Antibody (5G9/3) Alexa Fluor® 546 | sc-53437 AF546 | 200 µg/ml | $364.00 | |||
RuvC Antibody (5G9/3) Alexa Fluor® 594 | sc-53437 AF594 | 200 µg/ml | $364.00 | |||
RuvC Antibody (5G9/3) Alexa Fluor® 647 | sc-53437 AF647 | 200 µg/ml | $364.00 | |||
RuvC Antibody (5G9/3) Alexa Fluor® 680 | sc-53437 AF680 | 200 µg/ml | $364.00 | |||
RuvC Antibody (5G9/3) Alexa Fluor® 790 | sc-53437 AF790 | 200 µg/ml | $364.00 |