RUNX2 Double Nickase Plasmid (h): sc-400183-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RUNX2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RUNX2 Double Nickase Plasmid (h) and RUNX2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RUNX2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RUNX2 Antibody (F-2): sc-390351
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RUNX2 Double Nickase Plasmid (h) | sc-400183-NIC | 20 µg | $410.00 | |||
RUNX2 Double Nickase Plasmid (h2) | sc-400183-NIC-2 | 20 µg | $410.00 |
RUNX2 encodes runt-related transcription factor 2 (RUNX2), a master regulator of osteoblast lineage commitment and skeletal morphogenesis that binds RUNX consensus elements to coordinate gene programs controlling extracellular matrix production and mineralization. It integrates signaling from BMP/TGF-β, Wnt/β-catenin, MAPK, and hedgehog pathways to regulate differentiation, cell-cycle progression, and transcriptional networks in mesenchymal progenitors. RUNX2 activity shapes chromatin and cooperates with cofactors such as CBFB to drive expression of osteogenic genes including COL1A1, ALPL, and BGLAP. Dysregulated RUNX2 expression or function is implicated in developmental skeletal disorders, altered bone homeostasis, and context-dependent roles in tumor progression and metastasis through effects on EMT-associated transcriptional programs and matrix remodeling.
RUNX2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RUNX2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RUNX2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RUNX2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RUNX2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.