RUNX2 Antibody (C-12): sc-390715
- RUNX2 Antibody (C-12) is a mouse monoclonal IgG1 κ, cited in 51 publications, provided at 200 µg/ml
- raised against amino acids 294-363 of RUNX2 of mouse origin
- Anti-RUNX2 Antibody (C-12) is recommended for detection of RUNX2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine, porcine and canine
- Anti-RUNX2 Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-390715 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for RUNX2 Antibody (C-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with RUNX2 Antibody (C-12) (see ordering information below).
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RUNX2 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects RUNX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RUNX2 (C-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RUNX2, a member of the Runt-related transcription factor family, plays a critical role in skeletal development and mineralization by regulating osteoblast differentiation from mesenchymal stem cells. This transcription factor primarily resides in the nucleus, where RUNX2 binds to specific DNA sequences to activate or repress target genes essential for bone formation and remodeling. Nuclear localization of RUNX2 enables interaction with other transcription factors and co-regulators, orchestrating complex gene expression programs necessary for osteogenesis. RUNX2 dysregulation can lead to various skeletal disorders, including cleidocranial dysplasia syndrome, characterized by skeletal abnormalities such as hypoplastic clavicles and dental anomalies. Understanding RUNX2 nuclear localization and gene regulation roles helps researchers explore therapeutic strategies for enhancing bone formation and treating related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RUNX2 Antibody (C-12) References:
- Runx2 and microRNA regulation in bone and cartilage diseases. | Zhao, W., et al. 2016. Ann N Y Acad Sci. 1383: 80-87. PMID: 27526290
- Post-Translational Regulations of Transcriptional Activity of RUNX2. | Kim, HJ., et al. 2020. Mol Cells. 43: 160-167. PMID: 31878768
- Regulation of Runx2 by post-translational modifications in osteoblast differentiation. | Gomathi, K., et al. 2020. Life Sci. 245: 117389. PMID: 32007573
- A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation. | Kim, JM., et al. 2020. Nat Commun. 11: 2289. PMID: 32385263
- FTO/RUNX2 signaling axis promotes cementoblast differentiation under normal and inflammatory condition. | Sun, Q., et al. 2022. Biochim Biophys Acta Mol Cell Res. 1869: 119358. PMID: 36084732
- RUNX2 Reverses p53-Induced Chemotherapy Resistance in Gastric Cancer. | Huang, Y., et al. 2023. Pharmgenomics Pers Med. 16: 253-261. PMID: 37009416
- Glutathione limits RUNX2 oxidation and degradation to regulate bone formation. | Hu, G., et al. 2023. JCI Insight. 8: PMID: 37432749
- Shh-Gli2-Runx2 inhibits vascular calcification. | Huang, A., et al. 2024. Nephrol Dial Transplant. 39: 305-316. PMID: 37451818
- Runx2 deletion in hypertrophic chondrocytes impairs osteoclast mediated bone resorption. | Rashid, H., et al. 2024. Bone. 181: 117014. PMID: 38218304
- Runx2 Regulates Galnt3 and Fgf23 Expressions and Galnt3 Decelerates Osteoid Mineralization by Stabilizing Fgf23. | Jiang, Q., et al. 2024. Int J Mol Sci. 25: PMID: 38396954
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RUNX2 Antibody (C-12) | sc-390715 | 200 µg/ml | $322.00 | |||
RUNX2 Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-526186 | 200 µg Ab; 10 µg BP | $361.00 | |||
RUNX2 Antibody (C-12) X | sc-390715 X | 200 µg/0.1 ml | $322.00 | |||
RUNX2 Antibody (C-12) AC | sc-390715 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RUNX2 Antibody (C-12) HRP | sc-390715 HRP | 200 µg/ml | $322.00 | |||
RUNX2 Antibody (C-12) FITC | sc-390715 FITC | 200 µg/ml | $336.00 | |||
RUNX2 Antibody (C-12) PE | sc-390715 PE | 200 µg/ml | $349.00 | |||
RUNX2 Antibody (C-12) Alexa Fluor® 488 | sc-390715 AF488 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (C-12) Alexa Fluor® 546 | sc-390715 AF546 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (C-12) Alexa Fluor® 594 | sc-390715 AF594 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (C-12) Alexa Fluor® 647 | sc-390715 AF647 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (C-12) Alexa Fluor® 680 | sc-390715 AF680 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (C-12) Alexa Fluor® 790 | sc-390715 AF790 | 200 µg/ml | $364.00 |