RT1-B Antibody (HIS 19): sc-59359
- RT1-B Antibody (HIS 19) is a mouse monoclonal IgG1 κ RT1-B antibody provided at 200 µg/ml
- raised against Peyer's Patch lymphocytes of AO rat origin
- RT1-B Antibody (HIS 19) is recommended for detection of RT1-B of mouse and rat origin by WB, IP, IF, IHC(P) and FCM; non cross-reactive with RT1n
- Anti-RT1-B Antibody (HIS 19) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RT1-B Antibody (HIS 19) for WB and IHC(P) applications. These reagents are now offered in bundles with RT1-B Antibody (HIS 19) (see ordering information below).
RT1-B Antibody (HIS 19) is a mouse monoclonal IgG1 kappa light chain antibody that detects RT1-B protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FCM). RT1-B (HIS 19) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RT1-B protein plays a crucial role in immune response as part of the major histocompatibility complex (MHC) in rats, which is essential for presenting antigens to T cells. Specifically, RT1-B is a dimeric protein composed of an alpha and beta glycoprotein chain, homologous to I-A and I-E genes found in mouse H-2 complex. This structural configuration is vital for antigen presentation, as RT1-B allows for binding and display of peptide fragments derived from extracellular proteins, thereby facilitating T helper cell activation. Studies of MHC Class II antigens like RT1-B are significant for understanding T helper cell interactions with class II positive antigen-presenting cells, such as dendritic cells, B cells, and macrophages, while RT1-B antibodies also stain stromal cells in thymus, opening new avenues for investigating T helper cell development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RT1-B Antibody (HIS 19) References:
- Complementary DNA sequences encoding the rat MHC class II RT1-Bu and RT1-Du alpha and beta chains. | Easterfield, AJ., et al. 2003. Immunogenetics. 55: 344-50. PMID: 12879310
- Open reading frame sequencing and structure-based alignment of polypeptides encoded by RT1-Bb, RT1-Ba, RT1-Db, and RT1-Da alleles. | Ettinger, RA., et al. 2004. Immunogenetics. 56: 585-96. PMID: 15517241
- Considerable haplotypic diversity in the RT1-CE class I gene region of the rat major histocompatibility complex. | Roos, C. and Walter, L. 2005. Immunogenetics. 56: 773-7. PMID: 15578174
- Novel antibody tags from the rat lysosomal protein RT1.DM for immunodetection of recombinant proteins. | Neumann, J. 2005. J Immunol Methods. 301: 66-76. PMID: 15896798
- Complete coding nucleotide sequence of cDNA for the class II RT1.B beta I chain of the Lewis rat. | Syha-Jedelhauser, J., et al. 1991. Biochim Biophys Acta. 1089: 414-6. PMID: 1859846
- Polymorphism of the class II gene of rat major histocompatibility complex, RT1: partial sequence comparison of the first domain of the RT1.B beta 1 alleles. | Fujii, H., et al. 1991. Immunogenetics. 33: 399-403. PMID: 1904838
- Ir gene for bovine insulin in the rat maps to RT1.B beta. | Mizuno, K., et al. 1987. J Immunogenet. 14: 159-62. PMID: 3121753
- Elevated mRNA levels of major histocompatibility complex class II genes in lymphocytes of autoimmune BB rats. | Holowachuk, EW., et al. 1988. Diabetes. 37: 1637-40. PMID: 3142800
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RT1-B Antibody (HIS 19) | sc-59359 | 200 µg/ml | $322.00 | |||
RT1-B Antibody (HIS 19): m-IgG Fc BP-HRP Bundle | sc-526932 | 200 µg Ab; 10 µg BP | $361.00 | |||
RT1-B Antibody (HIS 19): m-IgG1 BP-HRP Bundle | sc-532305 | 200 µg Ab; 20 µg BP | $361.00 | |||
RT1-B Antibody (HIS 19) AC | sc-59359 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RT1-B Antibody (HIS 19) HRP | sc-59359 HRP | 200 µg/ml | $322.00 | |||
RT1-B Antibody (HIS 19) FITC | sc-59359 FITC | 200 µg/ml | $336.00 | |||
RT1-B Antibody (HIS 19) PE | sc-59359 PE | 200 µg/ml | $349.00 | |||
RT1-B Antibody (HIS 19) Alexa Fluor® 488 | sc-59359 AF488 | 200 µg/ml | $364.00 | |||
RT1-B Antibody (HIS 19) Alexa Fluor® 546 | sc-59359 AF546 | 200 µg/ml | $364.00 | |||
RT1-B Antibody (HIS 19) Alexa Fluor® 594 | sc-59359 AF594 | 200 µg/ml | $364.00 | |||
RT1-B Antibody (HIS 19) Alexa Fluor® 647 | sc-59359 AF647 | 200 µg/ml | $364.00 | |||
RT1-B Antibody (HIS 19) Alexa Fluor® 680 | sc-59359 AF680 | 200 µg/ml | $364.00 | |||
RT1-B Antibody (HIS 19) Alexa Fluor® 790 | sc-59359 AF790 | 200 µg/ml | $364.00 |