RT1-Ac Antibody (OX27): sc-53076
- RT1-Ac Antibody (OX27) is a mouse monoclonal IgG2a κ RT1-Ac antibody provided at 200 µg/ml
- raised against PHA activated lymphocytes of rat origin
- RT1-Ac Antibody (OX27) is recommended for detection of RT1-Ac of mouse and rat origin by IF and FCM
- Anti-RT1-Ac Antibody (OX27) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RT1-Ac Antibody (OX27). These reagents are now offered in bundles with RT1-Ac Antibody (OX27) (see ordering information below).
RT1-Ac Antibody (OX27) is a mouse monoclonal IgG2a kappa light chain antibody that detects RT1-Ac protein of mouse and rat origin by immunofluorescence (IF) and flow cytometry (FCM). Anti-RT1-Ac antibody (OX27) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RT1-A protein is a crucial component of the rat class I major histocompatibility complex (MHC I), which plays a vital role in the immune response by presenting nonself peptides on the cell surface for recognition by cytotoxic T lymphocytes (CTLs). Structurally, RT1-A molecules consist of two polypeptide chains: an alpha or heavy chain and beta-2-microglobulin, which is a non-covalently associated protein. This unique structure allows RT1-A to effectively bind and present antigenic peptides, typically 8-10 residues in length, within a specialized peptide binding groove. The ability of RT1-Ac to present these peptides is essential for the activation of CTLs, which are critical for the immune system′s ability to identify and eliminate infected or malignant cells. RT1-Ac represents haplotype c of the RT1-A antigens, making RT1-Ac (OX27) antibody a valuable tool for studying immune responses in both mouse and rat models.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RT1-Ac Antibody (OX27) References:
- Two different, highly exposed, bulged structures for an unusually long peptide bound to rat MHC class I RT1-Aa. | Speir, JA., et al. 2001. Immunity. 14: 81-92. PMID: 11163232
- Pristane, a non-antigenic adjuvant, induces MHC class II-restricted, arthritogenic T cells in the rat. | Holmberg, J., et al. 2006. J Immunol. 176: 1172-9. PMID: 16394006
- Immune responses to noninherited maternal RT1A antigens in inbred rats. | Propper, DJ., et al. 1991. Transplantation. 52: 331-5. PMID: 1871807
- The alloantibody response to semiallogeneic pregnancy in the rat. II. Antibodies in serum directed to multiple epitopes on conventional RT1A antigens. | Power, DA., et al. 1988. Transplantation. 46: 413-8. PMID: 2458642
- Hemorrhagic autoimmune encephalomyelitis induced by adoptive transfer of activated semiallogeneic spleen cells into irradiated rats. | Matsumoto, Y., et al. 1988. Am J Pathol. 133: 306-15. PMID: 2461090
- The alloantibody response to semiallogeneic pregnancy in the rat. I. Alloantibodies in sera and placental eluates directed to RT1A antigens. | Innes, A., et al. 1988. Transplantation. 46: 409-13. PMID: 3420685
- Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. | Fukumoto, T., et al. 1982. Eur J Immunol. 12: 237-43. PMID: 6178598
- An enzyme immunoassay for rat soluble MHC class I molecules (RT1a) and the release of soluble class I from mitogenically stimulated mononuclear cells. | Pockley, AG., et al. 1995. Immunol Invest. 24: 679-87. PMID: 8543333
- The rat MHC haplotype RT1c expresses two classical class I molecules. | Joly, E., et al. 1996. J Immunol. 157: 1551-8. PMID: 8759738
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RT1-Ac Antibody (OX27) | sc-53076 | 200 µg/ml | $322.00 | |||
RT1-Ac Antibody (OX27): m-IgG Fc BP-HRP Bundle | sc-525574 | 200 µg Ab; 10 µg BP | $361.00 | |||
RT1-Ac Antibody (OX27): m-IgG2a BP-HRP Bundle | sc-546321 | 200 µg Ab; 10 µg BP | $361.00 | |||
RT1-Ac Antibody (OX27) AC | sc-53076 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RT1-Ac Antibody (OX27) HRP | sc-53076 HRP | 200 µg/ml | $322.00 | |||
RT1-Ac Antibody (OX27) FITC | sc-53076 FITC | 200 µg/ml | $336.00 | |||
RT1-Ac Antibody (OX27) PE | sc-53076 PE | 200 µg/ml | $349.00 | |||
RT1-Ac Antibody (OX27) Alexa Fluor® 488 | sc-53076 AF488 | 200 µg/ml | $364.00 | |||
RT1-Ac Antibody (OX27) Alexa Fluor® 546 | sc-53076 AF546 | 200 µg/ml | $364.00 | |||
RT1-Ac Antibody (OX27) Alexa Fluor® 594 | sc-53076 AF594 | 200 µg/ml | $364.00 | |||
RT1-Ac Antibody (OX27) Alexa Fluor® 647 | sc-53076 AF647 | 200 µg/ml | $364.00 | |||
RT1-Ac Antibody (OX27) Alexa Fluor® 680 | sc-53076 AF680 | 200 µg/ml | $364.00 | |||
RT1-Ac Antibody (OX27) Alexa Fluor® 790 | sc-53076 AF790 | 200 µg/ml | $364.00 |