RSU1 Double Nickase Plasmid (h): sc-405511-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RSU1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RSU1 Double Nickase Plasmid (h) and RSU1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RSU1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RSU1 Antibody (1C6): sc-517140
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RSU1 Double Nickase Plasmid (h) | sc-405511-NIC | 20 µg | $410.00 | |||
RSU1 Double Nickase Plasmid (h2) | sc-405511-NIC-2 | 20 µg | $410.00 |
RSU1 (Ras Suppressor 1) is a focal adhesion–associated adaptor protein that binds PINCH/ILK complexes and helps couple integrin signaling to cytoskeletal organization. Through regulation of cell adhesion, spreading, and migration, RSU1 influences actin dynamics and downstream signaling nodes frequently linked to MAPK and PI3K/AKT pathway outputs. Perturbation of RSU1-mediated adhesion signaling has been associated with altered cell motility and survival programs relevant to tumor progression and invasion phenotypes. RSU1 is therefore widely studied in contexts such as extracellular matrix sensing, mechanotransduction, and adhesion-dependent control of proliferation and apoptosis.
RSU1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RSU1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RSU1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RSU1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RSU1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.