Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RRS1 CRISPR/Cas9 KO Plasmid (h): sc-409839
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RRS1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RRS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RRS1 Antibody (H-5): sc-515462
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RRS1 CRISPR/Cas9 KO Plasmid (h) | sc-409839 | 20 µg | $397.00 |
Overview
RRS1 (ribosome biogenesis regulator homolog) is a conserved nucleolar protein essential for 60S ribosomal subunit maturation and export through its roles in pre-rRNA processing and assembly of large subunit precursors. It interfaces with ribosome biogenesis factors and contributes to nucleolar organization, linking translational capacity to cell growth and cell-cycle progression. Disruption of ribosome production can activate nucleolar stress signaling and alter p53-dependent checkpoints, making RRS1 a useful node for studying proteostasis and growth control. Aberrant regulation of ribosome biogenesis pathways, including components such as RRS1, is frequently associated with proliferative phenotypes and is relevant for mechanistic studies in cancer biology and related ribosomopathies.
RRS1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RRS1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RRS1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RRS1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RRS1 protein expression.
This CRISPR knockout system enables efficient generation of RRS1-deficient cell models for investigation of RRS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RRS1 exon(s) critical for RRS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RRS1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RRS1 CRISPR/Cas9 KO Plasmid (h) and RRS1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RRS1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RRS1 HDR Plasmid (h) and RRS1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RRS1 homology arms to support homology-directed repair at defined RRS1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.