RRBP1 CRISPR/Cas9 KO Plasmid (h): sc-410995
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RRBP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RRBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RRBP1 CRISPR/Cas9 KO Plasmid (h) | sc-410995 | 20 µg | $397.00 |
Overview
RRBP1 (ribosome binding protein 1) encodes an endoplasmic reticulum (ER) membrane–associated protein that supports co-translational targeting of nascent polypeptides to the ER and contributes to ER organization and secretory pathway capacity. By linking ribosomes to the rough ER, RRBP1 influences protein synthesis, folding, and trafficking, processes tightly connected to ER homeostasis and the unfolded protein response. Altered RRBP1 expression has been reported in contexts of cellular stress and proliferative remodeling, making it relevant for studying proteostasis, ER-associated adaptation, and signaling networks coupled to secretory demand. RRBP1 is therefore of interest in models examining ER stress sensitivity, membrane trafficking dynamics, and disease-associated perturbations of protein handling.
RRBP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RRBP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RRBP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RRBP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RRBP1 protein expression.
This CRISPR knockout system enables efficient generation of RRBP1-deficient cell models for investigation of RRBP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RRBP1 exon(s) critical for RRBP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RRBP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RRBP1 CRISPR/Cas9 KO Plasmid (h) and RRBP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RRBP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RRBP1 HDR Plasmid (h) and RRBP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RRBP1 homology arms to support homology-directed repair at defined RRBP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.