RPTPα Antibody (H-4): sc-398203
- RPTPα Antibody (H-4) is a mouse monoclonal IgG2a κ RPTPα antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 178-223 within an internal region of RPTPα of human origin
- RPTP alpha Antibody (H-4) is recommended for detection of RPTPα of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-RPTP alpha Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RPTPα Antibody (H-4) for WB applications. These reagents are now offered in bundles with RPTPα Antibody (H-4) (see ordering information below).
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RPTPα Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects RPTP alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RPTPα antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The receptor protein-tyrosine phosphatase α (RPTPα) plays a crucial role in cellular signaling by dephosphorylating and activating Src family tyrosine kinases, which are essential for the regulation of integrin signaling, cell adhesion, and growth factor responsiveness. RPTPα is primarily located in the cell membrane, where RPTPα contains an extracellular domain, a single transmembrane segment, and two tandem intracytoplasmic catalytic domains, allowing RPTPα to form constitutive dimers that are vital for function. The human RPTPα sequence encodes a 793 amino acid protein, and in mouse models, RPTPα is constitutively phosphorylated at Ser 180 and Ser 204, which may influence activity and interactions. Additionally, RPTPα serves as a receptor for the Helicobacter pylori vacuolating cytotoxin, VacA, highlighting RPTPα′s importance in both normal cellular processes and pathogenic interactions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RPTPα Antibody (H-4) References:
- Multiple interactions between receptor protein-tyrosine phosphatase (RPTP) alpha and membrane-distal protein-tyrosine phosphatase domains of various RPTPs. | Blanchetot, C. and den Hertog, J. 2000. J Biol Chem. 275: 12446-52. PMID: 10777529
- Expression of protein tyrosine phosphatase alpha (RPTPalpha) in human breast cancer correlates with low tumor grade, and inhibits tumor cell growth in vitro and in vivo. | Ardini, E., et al. 2000. Oncogene. 19: 4979-87. PMID: 11042685
- Redox-regulated rotational coupling of receptor protein-tyrosine phosphatase alpha dimers. | van der Wijk, T., et al. 2003. J Biol Chem. 278: 13968-74. PMID: 12582170
- Protein-tyrosine phosphatase alpha, RPTP alpha, is a Helicobacter pylori VacA receptor. | Yahiro, K., et al. 2003. J Biol Chem. 278: 19183-9. PMID: 12626515
- RPTP-alpha acts as a transducer of mechanical force on alphav/beta3-integrin-cytoskeleton linkages. | von Wichert, G., et al. 2003. J Cell Biol. 161: 143-53. PMID: 12682088
- The receptor-like protein-tyrosine phosphatase, RPTP alpha, is phosphorylated by protein kinase C on two serines close to the inner face of the plasma membrane. | Tracy, S., et al. 1995. J Biol Chem. 270: 10587-94. PMID: 7537734
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RPTPα Antibody (H-4) | sc-398203 | 200 µg/ml | $316.00 | |||
RPTPα Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-526297 | 200 µg Ab; 10 µg BP | $354.00 | |||
RPTPα Antibody (H-4): m-IgG2a BP-HRP Bundle | sc-546570 | 200 µg Ab; 10 µg BP | $354.00 | |||
RPTPα Antibody (H-4) AC | sc-398203 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RPTPα Antibody (H-4) HRP | sc-398203 HRP | 200 µg/ml | $316.00 | |||
RPTPα Antibody (H-4) FITC | sc-398203 FITC | 200 µg/ml | $330.00 | |||
RPTPα Antibody (H-4) PE | sc-398203 PE | 200 µg/ml | $343.00 | |||
RPTPα Antibody (H-4) Alexa Fluor® 488 | sc-398203 AF488 | 200 µg/ml | $357.00 | |||
RPTPα Antibody (H-4) Alexa Fluor® 546 | sc-398203 AF546 | 200 µg/ml | $357.00 | |||
RPTPα Antibody (H-4) Alexa Fluor® 594 | sc-398203 AF594 | 200 µg/ml | $357.00 | |||
RPTPα Antibody (H-4) Alexa Fluor® 647 | sc-398203 AF647 | 200 µg/ml | $357.00 | |||
RPTPα Antibody (H-4) Alexa Fluor® 680 | sc-398203 AF680 | 200 µg/ml | $357.00 | |||
RPTPα Antibody (H-4) Alexa Fluor® 790 | sc-398203 AF790 | 200 µg/ml | $357.00 | |||
RPTPα (H-4) Neutralizing Peptide | sc-398203 P | 100 µg/0.5 ml | $68.00 |