Rpp25 Antibody (A-3): sc-514323
- Rpp25 Antibody (A-3) is a mouse monoclonal IgG1 κ Rpp25 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-21 at the N-terminus of Rpp25 of human origin
- Rpp25 Antibody (A-3) is recommended for detection of Rpp25 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Rpp25 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Rpp25 Antibody (A-3) for WB applications. These reagents are now offered in bundles with Rpp25 Antibody (A-3) (see ordering information below).
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Rpp25 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Rpp25 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rpp25 antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rpp25, a 199 amino acid nuclear protein, is a crucial component of the ribonuclease P and ribonuclease MRP ribonucleoproteins, which are essential for RNA processing. These ribonucleoproteins play a vital role in the maturation of precursor tRNA molecules by removing their 5-prime leader sequences, a process that is critical for the proper functioning of tRNA in protein synthesis. Rpp25 is encoded by a gene located on human chromosome 15q24.2 and mouse chromosome 9 B, and Rpp25′s interaction with other protein subunits, such as POP1 and POP5, highlights its importance in the assembly and function of the ribonuclease complexes. The accumulation of Rpp25 in the nucleolus underscores its significance in cellular processes, making Rpp25 (A-3) antibody an invaluable tool for researchers studying RNA metabolism and related pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rpp25 Antibody (A-3) References:
- Architecture and function of the human endonucleases RNase P and RNase MRP. | van Eenennaam, H., et al. 2000. IUBMB Life. 49: 265-72. PMID: 10995027
- Protein-RNA interactions in the subunits of human nuclear RNase P. | Jiang, T., et al. 2001. RNA. 7: 937-41. PMID: 11455963
- Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association. | van Eenennaam, H., et al. 2001. Mol Biol Cell. 12: 3680-9. PMID: 11694598
- Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P. | Guerrier-Takada, C., et al. 2002. RNA. 8: 290-5. PMID: 12003489
- Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex. | Welting, TJ., et al. 2004. Nucleic Acids Res. 32: 2138-46. PMID: 15096576
- cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein. | Roberts, JD., et al. 2007. Am J Physiol Lung Cell Mol Physiol. 293: L903-12. PMID: 17601797
- Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. | Jarrous, N., et al. 1998. RNA. 4: 407-17. PMID: 9630247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rpp25 Antibody (A-3) | sc-514323 | 200 µg/ml | $322.00 | |||
Rpp25 Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-527885 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rpp25 Antibody (A-3): m-IgG1 BP-HRP Bundle | sc-533258 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rpp25 Antibody (A-3) AC | sc-514323 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rpp25 Antibody (A-3) HRP | sc-514323 HRP | 200 µg/ml | $322.00 | |||
Rpp25 Antibody (A-3) FITC | sc-514323 FITC | 200 µg/ml | $336.00 | |||
Rpp25 Antibody (A-3) PE | sc-514323 PE | 200 µg/ml | $349.00 | |||
Rpp25 Antibody (A-3) Alexa Fluor® 488 | sc-514323 AF488 | 200 µg/ml | $364.00 | |||
Rpp25 Antibody (A-3) Alexa Fluor® 546 | sc-514323 AF546 | 200 µg/ml | $364.00 | |||
Rpp25 Antibody (A-3) Alexa Fluor® 594 | sc-514323 AF594 | 200 µg/ml | $364.00 | |||
Rpp25 Antibody (A-3) Alexa Fluor® 647 | sc-514323 AF647 | 200 µg/ml | $364.00 | |||
Rpp25 Antibody (A-3) Alexa Fluor® 680 | sc-514323 AF680 | 200 µg/ml | $364.00 | |||
Rpp25 Antibody (A-3) Alexa Fluor® 790 | sc-514323 AF790 | 200 µg/ml | $364.00 | |||
Rpp25 (A-3) Neutralizing Peptide | sc-514323 P | 100 µg/0.5 ml | $69.00 |