Date published: 2025-11-4
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RPA 32 kDa subunit Antibody (9H8) - Western Blotting - Image 19886
RPA 32 kDa 亚基抗体 (9H8): sc-56770. H69AR (A), HeLa (B), Saos-2 (C), T-47D (D) 和 MCF7 (E) 全细胞裂解液中 RPA 32 kDa 亚基表达的 Western 印迹分析.
RPA 32 kDa 亚基抗体 (9H8): sc-56770. H69AR (A), HeLa (B), Saos-2 (C), T-47D (D) 和 MCF7 (E) 全细胞裂解液中 RPA 32 kDa 亚基表达的 Western 印迹分析.

RPA 32 kDa subunit 抗体 (9H8): sc-56770

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说明书
  • RPA 32 kDa subunit 抗体 9H8 是小鼠单克隆 IgG1 κ，RPA 32 kDa subunit抗体, 在43篇文献中引用，规格为200 µg/ml
  • 针对全长RPA 32 kDa subunit，human起源
  • RPA 32 kDa subunit 抗体 (9H8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 RPA 32 kDa subunit
  • 抗RPA 32 kDa subunit抗体（9H8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是RPA 32 kDa subunit Antibody (9H8) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与RPA 32 kDa subunit Antibody (9H8) 打包提供（请参阅下面的订购信息）。

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描述
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研究信息

関連項目

附加Replication Protein A抗体，包括RPA 14 kDa subunit, RPA 32 kDa subunit和RPA 70 kDa subunit

RPA 32 kDa 亚基抗体 (9H8) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、石蜡包埋切片免疫组织化学 (IHCP) 和酶联免疫吸附试验 (ELISA) 检测小鼠、大鼠和人源的 RPA 32 kDa 亚基蛋白。RPA 32 kDa 亚基（9H8）抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物。RPA 32 kDa 亚基是复制蛋白 A（RPA）复合物的重要组成部分，对真核细胞中的 DNA 复制、重组和修复至关重要。RPA 32 kDa 亚基与细胞核中的单链 DNA 结合，稳定 DNA 并防止二级结构妨碍复制和修复过程。RPA 32 kDa 亚基的核定位允许在关键的细胞过程中有效地访问 DNA，确保基因组的稳定性和完整性。RPA 32 kDa 亚基与参与 DNA 修复的各种蛋白质（包括 UNG2、XPA 和 Rad52）相互作用，协调细胞对 DNA 损伤的反应。RPA 32 kDa 亚基与 Stat3 的 SH2 结构域特异性结合，表明它参与了调节细胞生长和存活的信号通路，这使得 RPA 32 kDa 亚基在 DNA 维护和细胞信号传导方面都具有重要意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RPA 32 kDa subunit 抗体 (9H8) 参考文献:

  1. 与 RFA1 基因相互作用的特征：RPA 在 DNA 复制和端粒维持中的作用。  |  Smith, J., et al. 2000. Biochimie. 82: 71-8. PMID: 10717390
  2. RPA 通过蛋白质与蛋白质之间的相互作用，稳定 XPA 破坏的 DNA 复合物。  |  Wang, M., et al. 2000. Biochemistry. 39: 6433-9. PMID: 10828957
  3. 复制蛋白 a 32 kDa 亚基（RPA p32）与 STAT3 的 SH2 结构域结合，并调节其转录活性。  |  Kim, J., et al. 2000. Cell Biol Int. 24: 467-73. PMID: 10875894
  4. 人类复制蛋白 A（hRPA）与伪原 DNA 底物结合的 5' --> 3' 分子极性。  |  Iftode, C. and Borowiec, JA. 2000. Biochemistry. 39: 11970-81. PMID: 11009611
  5. 复制因子 RPA 识别 DNA 修复蛋白 UNG2, XPA 和 RAD52 的结构基础。  |  Mer, G., et al. 2000. Cell. 103: 449-56. PMID: 11081631
  6. 编码人类复制蛋白 A 的 70 kDa 单链 DNA 结合亚基的 cDNA 的特征及其在 DNA 复制中的作用。  |  Erdile, LF., et al. 1991. J Biol Chem. 266: 12090-8. PMID: 2050703
  7. 人类复制蛋白 A 32 kDa 亚基的主要结构。  |  Erdile, LF., et al. 1990. J Biol Chem. 265: 3177-82. PMID: 2406247
  8. 与 DNA 结合的复制蛋白 A 单链 DNA 结合域的结构。  |  Bochkarev, A., et al. 1997. Nature. 385: 176-81. PMID: 8990123

订购信息

产品名称产品编号规格价格数量收藏夹

RPA 32 kDa subunit 抗体 (9H8)

sc-56770
200 µg/ml
$316.00

RPA 32 kDa subunit (9H8): m-IgG Fc BP-HRP 套装

sc-528620
200 µg Ab; 10 µg BP
$354.00

RPA 32 kDa subunit (9H8): m-IgGκ BP-HRP 套装

sc-521044
200 µg Ab, 40 µg BP
$354.00

RPA 32 kDa subunit (9H8): m-IgG1 BP-HRP 套装

sc-543032
200 µg Ab; 20 µg BP
$354.00

RPA 32 kDa subunit 抗体 (9H8) AC

sc-56770 AC
500 µg/ml, 25% agarose
$416.00

RPA 32 kDa subunit 抗体 (9H8) HRP

sc-56770 HRP
200 µg/ml
$316.00

RPA 32 kDa subunit 抗体 (9H8) FITC

sc-56770 FITC
200 µg/ml
$330.00

RPA 32 kDa subunit 抗体 (9H8) PE

sc-56770 PE
200 µg/ml
$343.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 488

sc-56770 AF488
200 µg/ml
$357.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 546

sc-56770 AF546
200 µg/ml
$357.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 594

sc-56770 AF594
200 µg/ml
$357.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 647

sc-56770 AF647
200 µg/ml
$357.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 680

sc-56770 AF680
200 µg/ml
$357.00

RPA 32 kDa subunit 抗体 (9H8) Alexa Fluor® 790

sc-56770 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with RPA 32 kDa subunit Antibody (9H8): sc-56770?

Asked by: jenniferc
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-10
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Rated 5 out of 5 by from Excellent antibody for IFI used this antibody at a 1:200 dilution on pancreatic cancer cell lines. It works well and gives repeatable results in IF. I haven't used it for Western blot yet.
Date published: 2023-08-03
Rated 5 out of 5 by from Works great for western blotThis is one of our commonly used antibody that works really well, especially as a control following probing with other phospho-specific RPA antibodies on western blot. Working dilution 1:6000 - 1:8000 depending on the amount of protein loaded.
Date published: 2021-11-04
Rated 5 out of 5 by from RPA32 (9H8) antibodyWorked very well for western blot. The signal was strong and specific.
Date published: 2019-04-09
Rated 5 out of 5 by from Great as loading controlThis antibody works very well. I have used it as loading control diluted 1:1000 in 5% skimmed milk.
Date published: 2018-10-29
Rated 4 out of 5 by from works well for IFgood detection of RPA32 foci in HEp-2 cells after etoposide treatment however for good detection, the antibody has to be used at 1:75 dilution in BSA for immunofluorescence staining
Date published: 2018-08-01
Rated 5 out of 5 by from Fantastic for WBWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 5 out of 5 by from Good feature!A strong band corresponding to topBP1 expression was detected with this antibody from the whole cell extract of Hela cells.
Date published: 2017-08-25
Rated 5 out of 5 by from Good antibody for western blotThe antibody detects a single band of the right size from HuH7 cell lysates at a 1:8000X dilution. It may work with an even higher dilution ratio.
Date published: 2017-07-26
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RPA 32 kDa subunit 抗体 (9H8) is rated 4.8 out of 5 by 16.
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