RP58 CRISPR/Cas9 KO Plasmid (h): sc-407058
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RP58 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RP58 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RP58 CRISPR/Cas9 KO Plasmid (h) | sc-407058 | 20 µg | $397.00 |
Overview
ZBTB18 encodes the zinc finger and BTB domain transcriptional repressor RP58, a nuclear regulator that modulates gene expression programs governing neural development, cell fate specification, and differentiation. RP58 contributes to chromatin-associated transcriptional control by engaging corepressor machinery and shaping lineage-specific transcriptional networks that influence proliferation and maturation. In human cells, altered ZBTB18/RP58 activity has been linked to dysregulated neurodevelopmental processes and aberrant transcriptional states observed in disease contexts, making it relevant for studies of gene regulatory architecture. Its downstream effects intersect with pathways controlling neuronal maturation, cell-cycle dynamics, and epigenetic programming.
RP58 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZBTB18 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZBTB18 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZBTB18 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RP58 protein expression.
This CRISPR knockout system enables efficient generation of ZBTB18-deficient cell models for investigation of RP58 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZBTB18 exon(s) critical for RP58 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZBTB18 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RP58 CRISPR/Cas9 KO Plasmid (h) and RP58 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZBTB18 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RP58 HDR Plasmid (h) and RP58 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZBTB18 homology arms to support homology-directed repair at defined ZBTB18 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.