RP1L1 CRISPR/Cas9 KO Plasmid (h): sc-405790
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RP1L1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RP1L1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RP1L1 CRISPR/Cas9 KO Plasmid (h) | sc-405790 | 20 µg | $397.00 |
Overview
RP1L1 (RP1-like 1) encodes a microtubule-associated protein enriched in photoreceptor cells, where it supports organization and stability of the axonemal cytoskeleton within the connecting cilium and outer segment. Through its association with microtubules and ciliary architecture, RP1L1 contributes to photoreceptor morphogenesis, outer segment disc alignment, and trafficking processes essential for phototransduction. Disruption of RP1L1 function is linked to inherited retinal degenerations, including macular dystrophy and related phenotypes, highlighting its role in maintaining retinal structure and visual signaling integrity. RP1L1 is therefore relevant for studying cilia-dependent cytoskeletal regulation, compartmentalized protein transport, and mechanisms of photoreceptor vulnerability.
RP1L1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RP1L1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RP1L1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RP1L1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RP1L1 protein expression.
This CRISPR knockout system enables efficient generation of RP1L1-deficient cell models for investigation of RP1L1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RP1L1 exon(s) critical for RP1L1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RP1L1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RP1L1 CRISPR/Cas9 KO Plasmid (h) and RP1L1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RP1L1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RP1L1 HDR Plasmid (h) and RP1L1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RP1L1 homology arms to support homology-directed repair at defined RP1L1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.