RoXaN CRISPR/Cas9 KO Plasmid (m): sc-422830
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RoXaN CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RoXaN genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RoXaN CRISPR/Cas9 KO Plasmid (m) | sc-422830 | 20 µg | $397.00 |
Overview
Zc3h7b encodes RoXaN, a zinc finger–containing protein implicated in RNA metabolism and post-transcriptional regulation, with reported roles in coordinating ribonucleoprotein complexes that influence mRNA stability and translation. Through these activities, RoXaN can affect cellular programs tied to proliferation, stress responses, and differentiation by shaping the expression of pathway components at the transcript level. Altered regulation of RNA-binding factors and zinc finger proteins is frequently linked to dysregulated growth control and immune or neurodevelopmental phenotypes, making Zc3h7b a useful locus for mechanistic studies of gene expression control. In mouse systems, Zc3h7b perturbation supports investigation of how RNA regulatory networks intersect with signaling and cell state transitions in disease-relevant contexts.
RoXaN CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Zc3h7b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Zc3h7b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Zc3h7b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RoXaN protein expression.
This CRISPR knockout system enables efficient generation of Zc3h7b-deficient cell models for investigation of RoXaN signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Zc3h7b exon(s) critical for RoXaN function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Zc3h7b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RoXaN CRISPR/Cas9 KO Plasmid (m) and RoXaN CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Zc3h7b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RoXaN HDR Plasmid (m) and RoXaN HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Zc3h7b homology arms to support homology-directed repair at defined Zc3h7b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.