Rotavirus Antibody (3F7): sc-53560
- Rotavirus Antibody (3F7) is a mouse monoclonal IgG2a κ Rotavirus antibody, cited in 1 publications, provided at 200 µg/ml
- raised against purified Rotavirus
- Rotavirus Antibody (3F7) is recommended for detection of Rotavirus by IP
- Anti-Rotavirus Antibody (3F7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rotavirus Antibody (3F7). These reagents are now offered in bundles with Rotavirus Antibody (3F7) (see ordering information below).
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Rotavirus Antibody (3F7) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Rotavirus protein of Rotavirus origin by immunoprecipitation (IP). Rotavirus (3F7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rotaviruses, which belong to the Reoviridae family, are characterized by their unique structure, featuring a genome composed of 11 segments of double-stranded RNA encased in a distinctive three-layered icosahedral protein capsid. This structural arrangement is crucial for the virus′s stability and ability to infect host cells. The robust capsid protects the viral RNA from degradation in the gastrointestinal tract, allowing the virus to effectively reach and penetrate the epithelial cells at the tips of the villi. Once inside, the viral proteins disrupt the endosomal membrane, leading to alterations in calcium ion concentrations that trigger significant changes in the epithelial cell structure, ultimately resulting in the symptoms associated with rotavirus infection, such as gastroenteritis, characterized by vomiting and watery diarrhea. Rotavirus (3F7) monoclonal antibody specifically targets the Rotavirus protein, making this reagent an invaluable tool for researchers studying the mechanisms of rotavirus pathogenesis and the development of potential therapeutic interventions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rotavirus Antibody (3F7) References:
- Relative localization of viroplasmic and endoplasmic reticulum-resident rotavirus proteins in infected cells. | González, RA., et al. 2000. Arch Virol. 145: 1963-73. PMID: 11043954
- Immunization with baculovirus-expressed recombinant rotavirus proteins VP1, VP4, VP6, and VP7 induces CD8+ T lymphocytes that mediate clearance of chronic rotavirus infection in SCID mice. | Dharakul, T., et al. 1991. J Virol. 65: 5928-32. PMID: 1656079
- Rotavirus proteins: structure and assembly. | Pesavento, JB., et al. 2006. Curr Top Microbiol Immunol. 309: 189-219. PMID: 16913048
- [Molecular drift of rotavirus during attenuation]. | Sergeev, VA., et al. 1987. Vopr Virusol. 32: 347-52. PMID: 2823483
- Myristylation of rotavirus proteins. | Clark, B. and Desselberger, U. 1988. J Gen Virol. 69 (Pt 10): 2681-6. PMID: 2844975
- Intracellular localization of rotaviral proteins. | Richardson, SC., et al. 1986. Arch Virol. 88: 251-64. PMID: 3010907
- Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. | Bastardo, JW., et al. 1981. Infect Immun. 34: 641-7. PMID: 6174448
- Identification of cytotoxic T cell epitopes on the VP3 and VP6 rotavirus proteins. | Franco, MA., et al. 1994. J Gen Virol. 75 (Pt 3): 589-96. PMID: 7510328
- Homotypic and heterotypic serum neutralizing antibody response to rotavirus proteins following natural primary infection and reinfection in children. | Gorrell, RJ. and Bishop, RF. 1999. J Med Virol. 57: 204-11. PMID: 9892409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rotavirus Antibody (3F7) | sc-53560 | 200 µg/ml | $316.00 | |||
Rotavirus Antibody (3F7): m-IgG Fc BP-HRP Bundle | sc-528574 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rotavirus Antibody (3F7): m-IgGκ BP-HRP Bundle | sc-520985 | 200 µg Ab, 40 µg BP | $354.00 | |||
Rotavirus Antibody (3F7): m-IgG2a BP-HRP Bundle | sc-547123 | 200 µg Ab; 10 µg BP | $354.00 | |||
Rotavirus Antibody (3F7) AC | sc-53560 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Rotavirus Antibody (3F7) HRP | sc-53560 HRP | 200 µg/ml | $316.00 | |||
Rotavirus Antibody (3F7) FITC | sc-53560 FITC | 200 µg/ml | $330.00 | |||
Rotavirus Antibody (3F7) PE | sc-53560 PE | 200 µg/ml | $343.00 | |||
Rotavirus Antibody (3F7) Alexa Fluor® 488 | sc-53560 AF488 | 200 µg/ml | $357.00 | |||
Rotavirus Antibody (3F7) Alexa Fluor® 546 | sc-53560 AF546 | 200 µg/ml | $357.00 | |||
Rotavirus Antibody (3F7) Alexa Fluor® 594 | sc-53560 AF594 | 200 µg/ml | $357.00 | |||
Rotavirus Antibody (3F7) Alexa Fluor® 647 | sc-53560 AF647 | 200 µg/ml | $357.00 | |||
Rotavirus Antibody (3F7) Alexa Fluor® 680 | sc-53560 AF680 | 200 µg/ml | $357.00 | |||
Rotavirus Antibody (3F7) Alexa Fluor® 790 | sc-53560 AF790 | 200 µg/ml | $357.00 |