Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RORγ Double Nickase Plasmid (h): sc-400912-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RORγ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RORγ Double Nickase Plasmid (h) and RORγ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RORC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RORγ Antibody (D-4): sc-365476
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RORγ Double Nickase Plasmid (h) | sc-400912-NIC | 20 µg | $410.00 | |||
RORγ Double Nickase Plasmid (h2) | sc-400912-NIC-2 | 20 µg | $410.00 |
RORC encodes the nuclear receptor RORγ, a ligand-regulated transcription factor that binds ROR response elements to control gene programs involved in immune differentiation and metabolism. RORγ is a central regulator of Th17 cell lineage commitment and IL-17 family cytokine expression, integrating signals from cytokine networks and nuclear receptor signaling to shape inflammatory transcriptional states. In addition to roles in adaptive immunity, RORγ contributes to lymphoid organogenesis and broader transcriptional control in hematopoietic cells. Dysregulated RORC/RORγ activity has been implicated in chronic inflammatory and autoimmune-associated pathways as well as tumor-immune microenvironment biology, making it a frequent target for mechanistic studies of immune signaling.
RORγ Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RORC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RORC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RORC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RORC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.