ROG CRISPR/Cas9 KO Plasmid (h): sc-406421
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ROG CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ROG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ROG CRISPR/Cas9 KO Plasmid (h) | sc-406421 | 20 µg | $397.00 |
Overview
ZBTB32 encodes the transcription factor ROG, a BTB/POZ domain zinc-finger protein that modulates gene expression programs controlling lymphocyte activation, differentiation, and effector function. ROG participates in transcriptional repression and chromatin-associated regulation of cytokine and activation-dependent genes, influencing pathways linked to immune homeostasis and antigen-driven responses. In human immune cells, altered ZBTB32 activity has been associated with dysregulated inflammatory signaling and context-dependent changes in T and B cell function, supporting its relevance to studies of immune-mediated disease mechanisms. As a regulatory node, ZBTB32 is frequently investigated in models of immune tolerance, immune exhaustion, and stimulus-responsive transcriptional remodeling.
ROG CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZBTB32 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZBTB32 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZBTB32 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ROG protein expression.
This CRISPR knockout system enables efficient generation of ZBTB32-deficient cell models for investigation of ROG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZBTB32 exon(s) critical for ROG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZBTB32 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ROG CRISPR/Cas9 KO Plasmid (h) and ROG CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZBTB32 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ROG HDR Plasmid (h) and ROG HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZBTB32 homology arms to support homology-directed repair at defined ZBTB32 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.