Rock-2 Double Nickase Plasmid (h): sc-400468-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rock-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Rock-2 Double Nickase Plasmid (h) and Rock-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ROCK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Rock-2 Antibody (D-11): sc-398519
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rock-2 Double Nickase Plasmid (h) | sc-400468-NIC | 20 µg | $410.00 | |||
Rock-2 Double Nickase Plasmid (h2) | sc-400468-NIC-2 | 20 µg | $410.00 |
Human ROCK2 encodes Rho-associated coiled-coil containing protein kinase 2 (Rock-2), a serine/threonine kinase that acts downstream of RhoA to control actomyosin contractility, stress fiber formation, focal adhesion dynamics, and cell polarity. ROCK2 signaling interfaces with cytoskeletal remodeling programs that influence cell migration, cytokinesis, and mechanotransduction, with downstream effects on myosin light chain phosphorylation and LIMK/cofilin-mediated actin turnover. Altered ROCK2 activity has been linked to dysregulated vascular tone and endothelial function, fibrotic responses driven by myofibroblast activation, and invasive phenotypes in tumor cell models. These pathway connections make ROCK2 a widely used node for studying Rho GTPase-dependent cytoskeletal regulation and context-specific signaling dependencies.
Rock-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ROCK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ROCK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ROCK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ROCK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.