robo4 Antibody (D-3): sc-166872

robo4 Antibody (D-3) is a mouse monoclonal IgM kappa antibody that detects robo4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-robo4 antibody (D-3) is available as the non-conjugated form. robo4 protein plays a crucial role in regulating angiogenesis and vascular development, acting as a key player in maintaining endothelial cell integrity and function. robo4 is primarily localized in endothelial cells, where robo4 modulates interaction between cells and extracellular matrix, influencing processes such as cell migration and proliferation. The unique structure of robo4, characterized by distinct extracellular domains, allows specific interaction with Slit ligands, which are essential for repelling axonal growth during neural development. This interaction contributes to proper formation of neural pathways and highlights robo4′s importance in vascular biology, helping prevent excessive cellular migration that could lead to pathological conditions. Using anti-robo4 antibody (D-3) in research can provide valuable insights into mechanisms of endothelial function and broader implications of robo4 in both neural and vascular development.

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robo4 Antibody (D-3) References:

1. Magic roundabout is a new member of the roundabout receptor family that is endothelial specific and expressed at sites of active angiogenesis.  |  Huminiecki, L., et al. 2002. Genomics. 79: 547-52. PMID: 11944987
2. Robo4 is a vascular-specific receptor that inhibits endothelial migration.  |  Park, KW., et al. 2003. Dev Biol. 261: 251-67. PMID: 12941633
3. Improving the comparative map of porcine chromosome 9 with respect to human chromosomes 1, 7 and 11.  |  Middleton, R., et al. 2003. Cytogenet Genome Res. 102: 128-32. PMID: 14970691
4. Soluble Robo4 receptor inhibits in vivo angiogenesis and endothelial cell migration.  |  Suchting, S., et al. 2005. FASEB J. 19: 121-3. PMID: 15486058
5. Magic roundabout, a tumor endothelial marker: expression and signaling.  |  Seth, P., et al. 2005. Biochem Biophys Res Commun. 332: 533-41. PMID: 15894287
6. Role of ROBO4 signalling in developmental and pathological angiogenesis.  |  Yadav, SS. and Narayan, G. 2014. Biomed Res Int. 2014: 683025. PMID: 24689049
7. ROBO4 variants predispose individuals to bicuspid aortic valve and thoracic aortic aneurysm.  |  Gould, RA., et al. 2019. Nat Genet. 51: 42-50. PMID: 30455415
8. Regulatory mechanisms of Robo4 and their effects on angiogenesis.  |  Dai, C., et al. 2019. Biosci Rep. 39: PMID: 31160487
9. Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions.  |  Adzraku, SY., et al. 2023. Cell Mol Biol Lett. 28: 2. PMID: 36647012
10. The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy.  |  Zhao, L., et al. 2023. J Transl Med. 21: 455. PMID: 37430272
11. Endothelial Robo4 suppresses endothelial-to-mesenchymal transition induced by irradiation and improves hematopoietic reconstitution.  |  Adzraku, SY., et al. 2024. Cell Death Dis. 15: 159. PMID: 38383474
12. Endothelial ROBO4 suppresses PTGS2/COX-2 expression and inflammatory diseases.  |  Tanaka, M., et al. 2024. Commun Biol. 7: 599. PMID: 38762541

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-robo4 Antibody (D-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-robo4 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

robo4 Antibody (D-3) Specifications

Alternate Product Name: robo4

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 955-974

Epitope Target: robo4

Epitope Species: human

Product Reactivity/Detection: robo4 isoforms 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for robo4 Antibody (D-3): P: Blocking Peptide