RNPS1 CRISPR/Cas9 KO Plasmid (m): sc-422692
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNPS1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RNPS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNPS1 CRISPR/Cas9 KO Plasmid (m) | sc-422692 | 20 µg | $397.00 |
Overview
Mouse Rnps1 encodes RNPS1, a conserved RNA-binding protein that functions in pre-mRNA splicing and post-splicing mRNP surveillance. RNPS1 participates in exon junction complex–associated processes and supports nonsense-mediated mRNA decay, influencing transcript quality control, mRNA export, and translation competence. Through these activities, RNPS1 helps maintain proteome integrity and regulates gene-expression programs linked to cell-cycle progression and stress responses. Dysregulation of splicing and mRNA surveillance pathways is broadly implicated in neurodevelopmental phenotypes and cancer-associated transcriptome remodeling, making RNPS1 a useful node for mechanistic studies of RNA processing.
RNPS1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rnps1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rnps1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rnps1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RNPS1 protein expression.
This CRISPR knockout system enables efficient generation of Rnps1-deficient cell models for investigation of RNPS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rnps1 exon(s) critical for RNPS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rnps1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RNPS1 CRISPR/Cas9 KO Plasmid (m) and RNPS1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rnps1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RNPS1 HDR Plasmid (m) and RNPS1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rnps1 homology arms to support homology-directed repair at defined Rnps1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.