RNMT Double Nickase Plasmid (h): sc-417964-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNMT Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNMT Double Nickase Plasmid (h) and RNMT Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNMT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RNMT Antibody (3H3-1D12): sc-517112
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNMT Double Nickase Plasmid (h) | sc-417964-NIC | 20 µg | $410.00 | |||
RNMT Double Nickase Plasmid (h2) | sc-417964-NIC-2 | 20 µg | $410.00 |
RNA guanine-7 methyltransferase (RNMT) is the catalytic subunit of the mRNA cap methyltransferase complex that installs the N7-methylguanosine (m7G) cap on RNA polymerase II transcripts. This cap modification is essential for co-transcriptional mRNA processing, nuclear export, translation initiation, and protection from exonucleolytic decay, linking RNMT activity to global control of gene expression programs. RNMT function interfaces with transcriptional and RNA processing pathways and helps coordinate growth and stress-responsive signaling through effects on transcript stability and translational output. Dysregulation of mRNA capping and cap-dependent translation has been associated with altered proliferative states and oncogenic gene-expression signatures, making RNMT a useful node for mechanistic studies of RNA metabolism in disease-relevant contexts.
RNMT Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNMT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNMT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNMT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNMT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.