RNMT Antibody (3H3-1D12): sc-517112

RNMT Antibody (3H3-1D12) is a mouse monoclonal IgG1 kappa light chain antibody that detects the RNMT protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-RNMT antibody (3H3-1D12) is available as the non-conjugated form. RNMT, or RNA (guanine-7-) methyltransferase, also known as MET, RG7MT1, or hCMT1c, is a crucial nuclear protein that plays a significant role in the mRNA capping process, which is essential for mRNA stability, processing, and translation. RNMT (3H3-1D12) monoclonal antibody recognizes a protein responsible for the final step in the addition of the m7GpppN cap to the 5′ end of mRNA, a modification that protects mRNA from degradation and facilitates its recognition by the ribosome during translation. The capping process involves three enzymatic steps, with RNMT catalyzing the transfer of a methyl group from S-adenosylmethionine to the GpppN end of the growing mRNA at the N-7 position, resulting in the production of S-adenosylhomocysteine and m7GpppN terminated RNA. Additionally, RNMT has two isoforms, hCMT1a and hCMT1b, generated through alternative splicing, which may have distinct regulatory roles in gene expression. RNMT (3H3-1D12) antibody provides researchers with a valuable tool for studying the dynamics of mRNA capping and its implications in various cellular processes and diseases.

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RNMT Antibody (3H3-1D12) References:

1. Cloning and characterization of mRNA capping enzyme and mRNA (Guanine-7-)-methyltransferase cDNAs from Xenopus laevis.  |  Yokoska, J., et al. 2000. Biochem Biophys Res Commun. 268: 617-24. PMID: 10679253
2. Cell-based assays to detect inhibitors of fungal mRNA capping enzymes and characterization of sinefungin as a cap methyltransferase inhibitor.  |  Chrebet, GL., et al. 2005. J Biomol Screen. 10: 355-64. PMID: 15964937
3. Amino acid residues within conserved domain VI of the vesicular stomatitis virus large polymerase protein essential for mRNA cap methyltransferase activity.  |  Li, J., et al. 2005. J Virol. 79: 13373-84. PMID: 16227259
4. Characterization of a Trypanosoma brucei RNA cap (guanine N-7) methyltransferase.  |  Hall, MP. and Ho, CK. 2006. RNA. 12: 488-97. PMID: 16431985
5. Genetic analysis of poxvirus mRNA cap methyltransferase: suppression of conditional mutations in the stimulatory D12 subunit by second-site mutations in the catalytic D1 subunit.  |  Schwer, B. and Shuman, S. 2006. Virology. 352: 145-56. PMID: 16716374
6. High-yield production of short GpppA- and 7MeGpppA-capped RNAs and HPLC-monitoring of methyltransfer reactions at the guanine-N7 and adenosine-2'O positions.  |  Peyrane, F., et al. 2007. Nucleic Acids Res. 35: e26. PMID: 17259217
7. Comparative expression analysis of isolated human adipocytes and the human adipose cell lines LiSa-2 and PAZ6.  |  van Beek, EA., et al. 2008. Int J Obes (Lond). 32: 912-21. PMID: 18283285
8. Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes.  |  Pillutla, RC., et al. 1998. J Biol Chem. 273: 21443-6. PMID: 9705270
9. Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively.  |  Pillutla, RC., et al. 1998. Genomics. 54: 351-3. PMID: 9828141

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNMT Antibody (3H3-1D12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-RNMT Antibody (3H3-1D12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNMT Antibody (3H3-1D12) Specifications

Alternate Product Name: RNMT

Product Clone Name: 3H3-1D12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-476

Epitope Target: RNMT

Epitope Species: human

Product Reactivity/Detection: RNMT

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for RNMT Antibody (3H3-1D12): N/A