RNH1 Antibody (D-4): sc-271724

RNH1 Antibody (D-4) is a mouse monoclonal IgG1 antibody that detects RNH1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RNH1, known as the placental ribonuclease inhibitor, is a crucial 460-amino acid protein characterized by a high content of leucine and cysteine residues, which contributes to structural stability and functional versatility. RNH1 plays a vital role in regulating mRNA turnover by binding to and inhibiting a wide range of intracellular and extracellular ribonucleases, effectively keeping them in a latent form. Notably, RNH1 also interacts with angiogenin, a member of the RNase A superfamily, thereby inhibiting angiogenesis—a process essential for tumor growth and metastasis. By modulating angiogenesis, RNH1 may have significant implications in cancer gene therapy, making RNH1 an important target for research in cancer biology and therapeutic development. Anti-RNH1 antibody (D-4) serves as an invaluable tool for scientists investigating the intricate roles of ribonuclease inhibitors in cellular processes and disease mechanisms.

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RNH1 Antibody (D-4) References:

1. A putative DEAD-box RNA-helicase is required for normal zoospore development in the late blight pathogen Phytophthora infestans.  |  Walker, CA., et al. 2008. Fungal Genet Biol. 45: 954-62. PMID: 18439859
2. The placental ribonuclease inhibitor (RNH) gene is located on chromosome subband 11p15.5.  |  Weremowicz, S., et al. 1990. Genomics. 8: 717-21. PMID: 2276743
3. RNH1 regulation of reactive oxygen species contributes to histone deacetylase inhibitor resistance in gastric cancer cells.  |  Zhu, Y., et al. 2014. Oncogene. 33: 1527-37. PMID: 23584480
4. Novel angiogenin mutants with increased cytotoxicity enhance the depletion of pro-inflammatory macrophages and leukemia cells ex vivo.  |  Cremer, C., et al. 2015. Cancer Immunol Immunother. 64: 1575-86. PMID: 26472728
5. Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor.  |  Thomas, SP., et al. 2018. RNA. 24: 1018-1027. PMID: 29748193
6. Enhancer-Driven lncRNA BDNF-AS Induces Endocrine Resistance and Malignant Progression of Breast Cancer through the RNH1/TRIM21/mTOR Cascade.  |  Lin, X., et al. 2020. Cell Rep. 31: 107753. PMID: 32521278
7. Human placental ribonuclease inhibitor abolishes both angiogenic and ribonucleolytic activities of angiogenin.  |  Shapiro, R. and Vallee, BL. 1987. Proc Natl Acad Sci U S A. 84: 2238-41. PMID: 3470787
8. APOBEC3B coordinates R-loop to promote replication stress and sensitize cancer cells to ATR/Chk1 inhibitors.  |  Zong, C., et al. 2023. Cell Death Dis. 14: 348. PMID: 37270643
9. The Antipsychotic Drug Aripiprazole Suppresses Colorectal Cancer by Targeting LAMP2a to Induce RNH1/miR-99a/mTOR-Mediated Autophagy and Apoptosis.  |  Hu, HF., et al. 2024. Adv Sci (Weinh). 11: e2409498. PMID: 39513392

The preferred RNH1 antibody is RNH1 Antibody (A-9): sc-365783.