RNGTT Antibody (D-3): sc-377464

RNGTT Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNGTT protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-RNGTT antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNGTT, also known as RNA guanylyltransferase and 5′-phosphatase, HCE, HCE1, hCAP, or CAP1A, is a 597 amino acid protein that plays a crucial role in mRNA processing by localizing to the nucleus and existing as four alternatively spliced isoforms. RNGTT is expressed in various tissues, including the kidney, lung, heart, brain, liver, testis, skin, and muscle, and functions as a bifunctional mRNA-capping enzyme. RNGTT exhibits RNA 5′-triphosphatase activity at its N-terminus and mRNA guanylyltransferase activity at its C-terminus, facilitating the initial steps of mRNA cap formation. Specifically, RNGTT removes a gamma-phosphate from the end of nascent mRNA and transfers the phospho residue to the 5′-diphosphate terminus, which is essential for mRNA stability and translation efficiency. The gene encoding RNGTT is located on human chromosome 6, which encompasses 170 million base pairs and constitutes nearly 6% of the human genome, highlighting the importance of RNGTT in cellular function and gene expression regulation.

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RNGTT Antibody (D-3) References:

1. Capping Enzyme mRNA-cap/RNGTT Regulates Hedgehog Pathway Activity by Antagonizing Protein Kinase A.  |  Chen, P., et al. 2017. Sci Rep. 7: 2891. PMID: 28588207
2. Structural insights into human co-transcriptional capping.  |  Garg, G., et al. 2023. Mol Cell. 83: 2464-2477.e5. PMID: 37369200
3. Temporary skin substitutes and evaporative water loss.  |  Moserová, J. and Bĕhounková-Housková, E. 1979. Scand J Plast Reconstr Surg. 13: 143-5. PMID: 377464
4. METTL3 alters capping enzyme expression and its activity on ribosomal proteins.  |  Del Valle-Morales, D., et al. 2023. bioRxiv.. PMID: 38045284
5. The mRNA-capping enzyme localizes to stress granules in the cytoplasm and maintains cap homeostasis of target mRNAs.  |  Gayen, A., et al. 2024. J Cell Sci. 137: PMID: 38841902
6. METTL3 alters capping enzyme expression and its activity on ribosomal proteins.  |  Del Valle-Morales, D., et al. 2024. Sci Rep. 14: 27720. PMID: 39532922
7. Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II.  |  Yue, Z., et al. 1997. Proc Natl Acad Sci U S A. 94: 12898-903. PMID: 9371772
8. Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme.  |  Tsukamoto, T., et al. 1998. Biochem Biophys Res Commun. 243: 101-8. PMID: 9473487
9. Isolation and characterization of a human cDNA for mRNA 5'-capping enzyme.  |  Yamada-Okabe, T., et al. 1998. Nucleic Acids Res. 26: 1700-6. PMID: 9512541
10. Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes.  |  Pillutla, RC., et al. 1998. J Biol Chem. 273: 21443-6. PMID: 9705270
11. Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase.  |  Tsukamoto, T., et al. 1998. Biochem Biophys Res Commun. 251: 27-34. PMID: 9790902
12. Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively.  |  Pillutla, RC., et al. 1998. Genomics. 54: 351-3. PMID: 9828141

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNGTT Antibody (D-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-RNGTT Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNGTT Antibody (D-3) Specifications

Alternate Product Name: RNGTT

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 57-89

Epitope Target: RNGTT

Epitope Species: human

Product Reactivity/Detection: RNGTT

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RNGTT Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred RNGTT antibody is RNGTT Antibody (H-3): sc-514128.