RNFT1 Antibody (10G3): sc-134430

RNFT1 Antibody (10G3) is a mouse monoclonal IgG2a kappa light chain antibody that detects RNFT1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-RNFT1 antibody (10G3) is available as a non-conjugated format. RNFT1, also known as RING finger and transmembrane domain-containing protein 1, plays a crucial role in the ubiquitination pathway, which is essential for protein degradation and cellular regulation. RNFT1 features a RING-type zinc finger motif, a conserved cysteine-rich domain capable of binding two zinc ions, which is significant for mediating protein-protein interactions and facilitating the ubiquitination process. RNFT1 is a multi-pass membrane protein consisting of 435 amino acids and exists in three alternatively spliced isoforms, highlighting its complexity and potential regulatory roles in various cellular processes. The gene encoding RNFT1 is located on human chromosome 17, a region that harbors key tumor suppressor genes such as p53 and BRCA1, both vital for maintaining genomic stability and preventing cancer. RNFT1 dysfunction may contribute to the dysregulation of these pathways, underscoring its importance in cancer biology and cellular homeostasis.

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RNFT1 Antibody (10G3) References:

1. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.  |  Lorick, KL., et al. 1999. Proc Natl Acad Sci U S A. 96: 11364-9. PMID: 10500182
2. p53 website and analysis of p53 gene mutations in human cancer: forging a link between epidemiology and carcinogenesis.  |  Soussi, T., et al. 2000. Hum Mutat. 15: 105-13. PMID: 10612830
3. Distinct pattern of p53 phosphorylation in human tumors.  |  Minamoto, T., et al. 2001. Oncogene. 20: 3341-7. PMID: 11423984
4. Three primary malignancies related to BRCA mutation successively occurring in a BRCA1 185delAG mutation carrier.  |  Piura, B., et al. 2001. Eur J Obstet Gynecol Reprod Biol. 97: 241-4. PMID: 11451557
5. Closing in on a breast cancer gene on chromosome 17q.  |  Hall, JM., et al. 1992. Am J Hum Genet. 50: 1235-42. PMID: 1598904
6. The RING finger. A novel protein sequence motif related to the zinc finger.  |  Freemont, PS. 1993. Ann N Y Acad Sci. 684: 174-92. PMID: 8317827
7. The RING finger domain: a recent example of a sequence-structure family.  |  Borden, KL. and Freemont, PS. 1996. Curr Opin Struct Biol. 6: 395-401. PMID: 8804826
8. A detailed study of loss of heterozygosity on chromosome 17 in tumours from Li-Fraumeni patients carrying a mutation to the TP53 gene.  |  Varley, JM., et al. 1997. Oncogene. 14: 865-71. PMID: 9047394
9. Loss of heterozygosity for defined regions on chromosomes 3, 11 and 17 in carcinomas of the uterine cervix.  |  Kersemaekers, AM., et al. 1998. Br J Cancer. 77: 192-200. PMID: 9460988