RNF8 Antibody (B-2): sc-271462

RNF8 Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects RNF8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-RNF8 antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNF8, also known as Ring Finger Protein 8, plays a crucial role in the ubiquitin-proteasome pathway, functioning as an E3 ubiquitin ligase that facilitates the ubiquitination of target proteins, which is essential for their degradation and regulation within the cell. This process is vital for maintaining cellular homeostasis and regulating various cellular functions, including DNA repair, cell cycle progression, and apoptosis. RNF8 is predominantly located in the nucleus, where RNF8 interacts with the heterodimeric ubiquitin-conjugating enzyme UBC13, co-localizing to promote polyubiquitination of substrates. Additionally, RNF8 has been shown to bind to Retinoid X receptor alpha (RXRα), enhancing RXRα transcriptional activity, which underscores RNF8′s importance in gene regulation. RNF8′s ability to modulate RXRα-mediated transcription further highlights RNF8′s role in cellular signaling pathways, making RNF8 a significant target for research in cancer and other diseases where ubiquitination processes are disrupted.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RNF8 Antibody (B-2) References:

1. The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1.  |  Moynihan, TP., et al. 1999. J Biol Chem. 274: 30963-8. PMID: 10521492
2. Interaction of the ring finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes.  |  Pringa, E., et al. 2001. J Biol Chem. 276: 19617-23. PMID: 11274149
3. N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8.  |  Ito, K., et al. 2001. Eur J Biochem. 268: 2725-32. PMID: 11322894
4. Protein-protein interactions within an E2-RING finger complex. Implications for ubiquitin-dependent DNA damage repair.  |  Ulrich, HD. 2003. J Biol Chem. 278: 7051-8. PMID: 12496280
5. Small nuclear RING finger protein expression during gonad development: regulation by gonadotropins and estrogen in the postnatal ovary.  |  Hirvonen-Santti, SJ., et al. 2004. Endocrinology. 145: 2433-44. PMID: 14749358
6. The RING finger protein, RNF8, interacts with retinoid X receptor alpha and enhances its transcription-stimulating activity.  |  Takano, Y., et al. 2004. J Biol Chem. 279: 18926-34. PMID: 14981089
7. The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation.  |  Plans, V., et al. 2006. J Cell Biochem. 97: 572-82. PMID: 16215985
8. The RING-finger protein haprin: domains and function in the acrosome reaction.  |  Kitamura, K., et al. 2005. Curr Protein Pept Sci. 6: 567-74. PMID: 16381605
9. How a RING finger protein and a steroid hormone control autophagy.  |  Lindmo, K. and Stenmark, H. 2006. Autophagy. 2: 321-2. PMID: 16874068

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNF8 Antibody (B-2) has 58 citations in a variety of scientific publications.

Primary antibodies like Anti-RNF8 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNF8 Antibody (B-2) Specifications

Alternate Product Name: E3 Ubiquitin-Protein Ligase

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 186-485

Epitope Target: RNF8

Epitope Species: human

Product Reactivity/Detection: RNF8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 58 citations in various scientific publications

Available Conjugates/Forms for RNF8 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin