RNF4 CRISPR/Cas9 KO Plasmid (m): sc-422689
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RNF4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF4 CRISPR/Cas9 KO Plasmid (m) | sc-422689 | 20 µg | $397.00 |
Overview
Rnf4 encodes RNF4, a RING-type SUMO-targeted ubiquitin ligase (STUbL) that recognizes polySUMOylated proteins and catalyzes their ubiquitination to direct turnover or remodeling of protein complexes. In mouse cells, RNF4 helps regulate DNA damage responses by promoting the clearance of SUMO-modified repair and signaling factors, linking SUMOylation to ubiquitin-dependent proteostasis. This activity contributes to genome stability, replication stress handling, and transcriptional control through dynamic regulation of nuclear protein assemblies. Dysregulation of STUbL-mediated pathways is broadly relevant to cellular transformation and neurodegenerative phenotypes associated with impaired protein quality control and defective DNA repair.
RNF4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rnf4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rnf4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rnf4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RNF4 protein expression.
This CRISPR knockout system enables efficient generation of Rnf4-deficient cell models for investigation of RNF4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rnf4 exon(s) critical for RNF4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rnf4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RNF4 CRISPR/Cas9 KO Plasmid (m) and RNF4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rnf4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RNF4 HDR Plasmid (m) and RNF4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rnf4 homology arms to support homology-directed repair at defined Rnf4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.