RNF4 Antibody (205F2G): sc-517643

RNF4 Antibody (205F2G) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNF4 protein of human origin by western blotting (WB). RNF4 Antibody (205F2G) is available as a non-conjugated anti-RNF4 antibody (205F2G). RNF4, also known as SNURF, plays a crucial role in cellular processes due to involvement in transcriptional regulation. RNF4 contains a C-terminal RING finger motif, a specialized zinc finger domain that facilitates interaction with various DNA structures, including linear, super-coiled, and four-way junction DNA. This ability to bind different forms of DNA allows RNF4 to influence nucleoprotein structure assembly, which is essential for proper gene expression and chromatin organization. RNF4 is predominantly expressed in human testis and mouse embryo, highlighting importance in developmental processes and germ cell function. RNF4 has been shown to interact with nucleosomes and can act as both a transcriptional activator and repressor, mediating androgen receptor activity and forming complexes with other proteins such as POZ-AT hook-zinc finger protein (PATZ) to regulate gene expression. The gene encoding human RNF4 is located on chromosome 4p16.3, underscoring relevance in genetic studies and potential implications in various diseases.

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RNF4 Antibody (205F2G) References:

1. A novel member of the BTB/POZ family, PATZ, associates with the RNF4 RING finger protein and acts as a transcriptional repressor.  |  Fedele, M., et al. 2000. J Biol Chem. 275: 7894-901. PMID: 10713105
2. The RING finger protein SNURF is a bifunctional protein possessing DNA binding activity.  |  Häkli, M., et al. 2001. J Biol Chem. 276: 23653-60. PMID: 11319220
3. RNF4 is a growth inhibitor expressed in germ cells but not in human testicular tumors.  |  Pero, R., et al. 2001. Am J Pathol. 159: 1225-30. PMID: 11583949
4. RNF4-Dependent Oncogene Activation by Protein Stabilization.  |  Thomas, JJ., et al. 2016. Cell Rep. 16: 3388-3400. PMID: 27653698
5. RNF4-mediated SUMO-targeted ubiquitination relieves PARIS/ZNF746-mediated transcriptional repression.  |  Nishida, T. and Yamada, Y. 2020. Biochem Biophys Res Commun. 526: 110-116. PMID: 32197837
6. RNF4~RGMb~BMP6 axis required for osteogenic differentiation and cancer cell survival.  |  Novak, R., et al. 2022. Cell Death Dis. 13: 820. PMID: 36153321
7. RNF4 prevents genomic instability caused by chronic DNA under-replication.  |  Oram, MK., et al. 2024. DNA Repair (Amst). 135: 103646. PMID: 38340377
8. RNF4 sustains Myc-driven tumorigenesis by facilitating DNA replication.  |  Her, J., et al. 2024. J Clin Invest. 134: PMID: 38530355
9. Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation.  |  Liu, JCY., et al. 2024. Nat Struct Mol Biol. 31: 1355-1367. PMID: 38649616
10. WRN inhibition leads to its chromatin-associated degradation via the PIAS4-RNF4-p97/VCP axis.  |  Rodríguez Pérez, F., et al. 2024. Nat Commun. 15: 6059. PMID: 39025847
11. Identification and preliminary characterization of a protein motif related to the zinc finger.  |  Lovering, R., et al. 1993. Proc Natl Acad Sci U S A. 90: 2112-6. PMID: 7681583
12. Identification and characterization of a novel RING-finger gene (RNF4) mapping at 4p16.3.  |  Chiariotti, L., et al. 1998. Genomics. 47: 258-65. PMID: 9479498