RNF20 Double Nickase Plasmid (h): sc-412695-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNF20 Double Nickase Plasmid (h) and RNF20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNF20. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RNF20 Antibody (1594CT552.128.36): sc-517358
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF20 Double Nickase Plasmid (h) | sc-412695-NIC | 20 µg | $410.00 | |||
RNF20 Double Nickase Plasmid (h2) | sc-412695-NIC-2 | 20 µg | $410.00 |
RNF20 encodes an E3 ubiquitin-protein ligase that, together with RNF40, forms the BRE1 complex responsible for monoubiquitination of histone H2B (H2BK120ub). This chromatin modification promotes transcriptional elongation and couples to downstream histone methylation programs, influencing genome-wide gene expression, DNA damage signaling, and repair pathway choice. RNF20 activity is linked to maintenance of genome stability through regulation of double-strand break responses and replication-associated chromatin remodeling. Dysregulation of RNF20-dependent histone ubiquitination has been associated with altered transcriptional networks and genomic instability observed across multiple disease contexts, supporting its study in epigenetic and DNA repair mechanisms.
RNF20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNF20 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNF20. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNF20 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNF20-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.