RNF149 CRISPR/Cas9 KO Plasmid (h): sc-406588
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF149 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RNF149 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF149 CRISPR/Cas9 KO Plasmid (h) | sc-406588 | 20 µg | $397.00 |
Overview
RNF149 encodes a RING finger–containing protein predicted to function as an E3 ubiquitin ligase, supporting ubiquitin-dependent control of protein stability and signaling dynamics. RING-type E3 ligases help regulate proteostasis, stress responses, and turnover of pathway components that shape cell-cycle progression and survival decisions. RNF149 is therefore relevant for dissecting how ubiquitination and proteasomal degradation coordinate cellular homeostasis and signal transduction. Altered ubiquitin-pathway regulation is frequently associated with tumor biology and other disorders linked to protein quality control, making RNF149 a useful target for mechanistic studies in disease-relevant models.
RNF149 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RNF149 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RNF149 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RNF149 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RNF149 protein expression.
This CRISPR knockout system enables efficient generation of RNF149-deficient cell models for investigation of RNF149 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RNF149 exon(s) critical for RNF149 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RNF149 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RNF149 CRISPR/Cas9 KO Plasmid (h) and RNF149 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RNF149 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RNF149 HDR Plasmid (h) and RNF149 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RNF149 homology arms to support homology-directed repair at defined RNF149 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.