Rnd1 CRISPR/Cas9 KO Plasmid (h): sc-406603
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Rnd1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Rnd1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rnd1 CRISPR/Cas9 KO Plasmid (h) | sc-406603 | 20 µg | $397.00 |
Overview
RND1 encodes Rnd1, an atypical Rho family small GTPase that is constitutively GTP-bound and functions primarily as a regulator of actin cytoskeleton organization and cell adhesion. Rnd1 signaling antagonizes RhoA/ROCK-dependent contractility, influencing stress fiber formation, focal adhesion dynamics, and cell rounding, with downstream effects on migration and neurite outgrowth. Through crosstalk with integrin signaling and cytoskeletal remodeling pathways, Rnd1 helps coordinate changes in cell morphology during development and tissue homeostasis. Altered RND1 expression or pathway imbalance has been associated with dysregulated motility and invasion phenotypes in cancer biology and with perturbations in neuronal and vascular processes studied in disease-relevant models.
Rnd1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RND1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RND1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RND1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Rnd1 protein expression.
This CRISPR knockout system enables efficient generation of RND1-deficient cell models for investigation of Rnd1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RND1 exon(s) critical for Rnd1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RND1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Rnd1 CRISPR/Cas9 KO Plasmid (h) and Rnd1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RND1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Rnd1 HDR Plasmid (h) and Rnd1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RND1 homology arms to support homology-directed repair at defined RND1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.