Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RNase L Double Nickase Plasmid (h): sc-403412-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNase L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNase L Double Nickase Plasmid (h) and RNase L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RNASEL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RNase L Antibody (E-9): sc-74405
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNase L Double Nickase Plasmid (h) | sc-403412-NIC | 20 µg | $410.00 | |||
RNase L Double Nickase Plasmid (h2) | sc-403412-NIC-2 | 20 µg | $410.00 |
Human RNASEL encodes RNase L, an interferon-inducible endoribonuclease activated by 2′-5′ oligoadenylate produced by OAS enzymes during innate immune sensing of viral and cellular double-stranded RNA. Once activated, RNase L cleaves single-stranded RNA to restrict pathogen replication, reshape the cellular transcriptome, and amplify antiviral signaling through pathways such as RIG-I/MDA5–MAVS and downstream type I interferon responses. RNase L activity also influences RNA stability, translation control, and stress responses, linking it to broader regulation of cell growth and apoptosis. Genetic variation or dysregulation in RNASEL has been associated with altered antiviral defense and inflammation-related phenotypes, supporting its relevance in studies of immune signaling and disease susceptibility mechanisms.
RNase L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RNASEL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RNASEL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RNASEL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RNASEL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.