RLIM Antibody (SK-46): sc-101117
- RLIM Antibody (SK-46) is a mouse monoclonal IgG2a κ RLIM antibody provided at 100 µg/ml
- raised against recombinant RLIM of human origin
- recommended for detection of RLIM of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for RLIM Antibody (SK-46). This work is in progress.
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RLIM Antibody (SK-46) is a mouse monoclonal IgG2a kappa light chain antibody that detects RLIM protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RLIM antibody (SK-46) is available as the non-conjugated form. RLIM protein, also known as RING finger LIM domain-binding protein or RNF12, is a 624 amino acid RING-H2 zinc finger protein that plays a crucial role in the ubiquitin-proteasome pathway, facilitating the ubiquitinylation and subsequent degradation of target proteins. This process is vital for maintaining cellular homeostasis and regulating various signaling pathways. RLIM is predominantly expressed in a variety of tissues and functions as a protein ligase that negatively regulates LIM homeodomain transcription factors by recruiting the Sin3A corepressor complex, which is essential for histone deacetylation. Additionally, RLIM interacts with CLIM proteins, which are positive co-regulators of LIM-HD transcription factors, leading to their ubiquitination and degradation. The structural integrity of RLIM, characterized by its RING-type zinc finger domain, is critical for its ligase activity and involvement in cellular processes, including implications in renal cell carcinoma.
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RLIM Antibody (SK-46) References:
- RLIM inhibits functional activity of LIM homeodomain transcription factors via recruitment of the histone deacetylase complex. | Bach, I., et al. 1999. Nat Genet. 22: 394-9. PMID: 10431247
- Functional characterization of the gene encoding RLIM, the corepressor of LIM homeodomain factors. | Ostendorff, HP., et al. 2000. Genomics. 69: 120-30. PMID: 11013082
- Selective degradation of excess Ldb1 by Rnf12/RLIM confers proper Ldb1 expression levels and Xlim-1/Ldb1 stoichiometry in Xenopus organizer functions. | Hiratani, I., et al. 2003. Development. 130: 4161-75. PMID: 12874135
- Dynamic expression of LIM cofactors in the developing mouse neural tube. | Ostendorff, HP., et al. 2006. Dev Dyn. 235: 786-91. PMID: 16395690
- RLIM interacts with Smurf2 and promotes TGF-β induced U2OS cell migration. | Huang, Y., et al. 2011. Biochem Biophys Res Commun. 414: 181-5. PMID: 21945933
- RLIM is dispensable for X-chromosome inactivation in the mouse embryonic epiblast. | Shin, J., et al. 2014. Nature. 511: 86-9. PMID: 24870238
- RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21. | Huang, Y., et al. 2017. Oncotarget. 8: 83075-83087. PMID: 29137325
- Rlim-Dependent and -Independent Pathways for X Chromosome Inactivation in Female ESCs. | Wang, F., et al. 2017. Cell Rep. 21: 3691-3699. PMID: 29281819
- Deficient spermiogenesis in mice lacking Rlim. | Wang, F., et al. 2021. Elife. 10: PMID: 33620316
- RLIM: a recursive and latent infection model for the prediction of US COVID-19 infections and turning points. | Yu, X., et al. 2021. Nonlinear Dyn. 106: 1397-1410. PMID: 34092919
- RLIM-specific activity reporters define variant pathogenicity in Tonne-Kalscheuer syndrome. | Bandi, V., et al. 2025. HGG Adv. 6: 100378. PMID: 39482882
- Localization of mRNAs for Rlim-1, the rat Xlim-1 homolog, in the developing rat brain. | Furuyama, T., et al. 1996. Brain Res Mol Brain Res. 36: 152-6. PMID: 9011751
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RLIM Antibody (SK-46) | sc-101117 | 100 µg/ml | $333.00 |