Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RKIP Double Nickase Plasmid (h): sc-401270-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RKIP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RKIP Double Nickase Plasmid (h) and RKIP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PEBP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RKIP Antibody (D-5): sc-365973
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RKIP Double Nickase Plasmid (h) | sc-401270-NIC | 20 µg | $410.00 | |||
RKIP Double Nickase Plasmid (h2) | sc-401270-NIC-2 | 20 µg | $410.00 |
Human PEBP1 encodes Raf kinase inhibitory protein (RKIP), a conserved modulator of signal transduction that restrains RAF1–MEK–ERK signaling and can influence additional kinase networks involved in proliferation, differentiation, and stress responses. RKIP also interfaces with GPCR kinase pathways and contributes to regulation of NF-κB–linked inflammatory signaling and apoptosis-related processes. Through these roles, RKIP helps shape cellular decisions between growth and death, with downstream effects on migration and epithelial–mesenchymal transition programs. Altered PEBP1/RKIP expression or activity has been associated with diverse pathological contexts, including cancer biology and neuroinflammatory or neurodegenerative mechanisms, making it a useful node for pathway dissection.
RKIP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PEBP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PEBP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PEBP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PEBP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.