RIP4 Antibody (E-7): sc-377368

RIP4 Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects RIP4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RIP4 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RIP4 protein, also known as receptor-interacting serine/threonine-protein kinase 4, plays a crucial role in cellular signaling pathways, particularly in the modulation of epidermal growth and differentiation. Located primarily in the cytoplasm, RIP4 interacts with tumor necrosis factor receptors (TNFRs) to facilitate downstream signaling that can lead to apoptosis, making RIP4 vital for maintaining cellular homeostasis and responding to stress signals. RIP4′s structure includes ten ankyrin repeats and a protein kinase domain, which are essential for its function in activating the NFκB signaling pathway, a key regulator of immune responses and cell survival. Additionally, alternative splicing of RIP4 results in two isoforms, further diversifying RIP4′s functional roles in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

RIP4 Antibody (E-7) References:

1. DIK, a novel protein kinase that interacts with protein kinase Cdelta. Cloning, characterization, and gene analysis.  |  Bähr, C., et al. 2000. J Biol Chem. 275: 36350-7. PMID: 10948194
2. RIP4 is an ankyrin repeat-containing kinase essential for keratinocyte differentiation.  |  Holland, P., et al. 2002. Curr Biol. 12: 1424-8. PMID: 12194825
3. RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis.  |  Meylan, E., et al. 2002. EMBO Rep. 3: 1201-8. PMID: 12446564
4. Protein kinase C-associated kinase can activate NFkappaB in both a kinase-dependent and a kinase-independent manner.  |  Moran, ST., et al. 2003. J Biol Chem. 278: 21526-33. PMID: 12676934
5. A catalytically inactive form of protein kinase C-associated kinase/receptor interacting protein 4, a protein kinase C beta-associated kinase that mediates NF-kappa B activation, interferes with early B cell development.  |  Cariappa, A., et al. 2003. J Immunol. 171: 1875-80. PMID: 12902489
6. Protein kinase C-associated kinase is not required for the development of peripheral B lymphocyte populations.  |  Moran, ST., et al. 2006. Mol Immunol. 43: 1694-9. PMID: 16256200
7. Identification of a novel putative mitogen-activated kinase cascade on human chromosome 21 by computational approaches.  |  Pellegrini-Calace, M. and Tramontano, A. 2006. Bioinformatics. 22: 775-8. PMID: 16428264
8. Regulation of NF-kappaB activity and keratinocyte differentiation by the RIP4 protein: implications for cutaneous wound repair.  |  Adams, S., et al. 2007. J Invest Dermatol. 127: 538-44. PMID: 17039240

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RIP4 Antibody (E-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-RIP4 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RIP4 Antibody (E-7) Specifications

Alternate Product Name: RIPK4

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 525-607

Epitope Target: RIP4

Epitope Species: human

Product Reactivity/Detection: RIP4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for RIP4 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin