Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RIP3 Double Nickase Plasmid (m): sc-425224-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RIP3 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RIP3 Double Nickase Plasmid (m) and RIP3 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ripk3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RIP3 Antibody (B-2): sc-374639
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RIP3 Double Nickase Plasmid (m) | sc-425224-NIC | 20 µg | $410.00 | |||
RIP3 Double Nickase Plasmid (m2) | sc-425224-NIC-2 | 20 µg | $410.00 |
Mouse Ripk3 encodes receptor-interacting serine/threonine-protein kinase 3 (RIP3), a central regulator of programmed necrosis (necroptosis) and inflammatory signaling. RIP3 cooperates with RIP1 and MLKL to assemble the necrosome, driving membrane disruption and release of damage-associated molecular patterns that amplify innate immune responses. This pathway intersects with TNF receptor signaling, TLR/TRIF pathways, and crosstalk with apoptosis and inflammasome activation, shaping cytokine production and cellular stress responses. Dysregulated RIP3 activity has been implicated in models of tissue injury and inflammation, including neuroinflammatory and metabolic phenotypes, making Ripk3 a common target for mechanistic studies of cell death and immune homeostasis.
RIP3 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ripk3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ripk3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ripk3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ripk3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.